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I4269

Sigma-Aldrich

Interleukin-4 human

IL-4, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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Synonym(s):
IL-4, hIL-4
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

≤0.400 ng/mL EC50

quality

endotoxin tested

mol wt

14.9 kDa (containing 129 amino acid residues)

packaging

pkg of 5 and 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.000 EU/μg

color

white

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... IL4(3565)
mouse ... IL4(3565)

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General description

Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including differentiation of naive T cells into the TH2 phenotype, promoting B cell proliferation, antibody isotype switching, and expression of other TH2 cytokines including IL-5 and IL-9.1,2 IL-4 plays a critical role in the development of allergic inflammation and asthma.3

IL-4 binds to two distinct receptors; the type I receptor, a heterodimer consisting of the IL-4Rα chain and the common gamma chain, γc, and the type II receptor, a heterodimer of IL-4Rα and IL-13Rα1.4

Naturally occurring IL-4 has a molecular mass of 12-20 kDa. Recombinant human IL-4 is a 14.9 kDa protein containing 129 amino acid residues. Human and mouse IL-4 share a 50% amino acid sequence homology, but their biological actions are species-specific.5

Application

Lymphokine with profound effects on the growth and differentiation of immunologically competent cells, inhibits VEGF-induced and βFGF-induced angiogenesis.

Physical form

Lyophilized from a sterile filtered solution.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of the proliferation of human TF-1 cells.

Citation

1. Paul, W.E., Interleukin-4: a prototypic immuno-regulatory lymphokine. Blood, 77, 1859-1870 (1991).
2. O′Garra, A., and Arai, N., The molecular basis of T helper 1 and T helper 2 cell differentiation. Trends Cell Biol., 10, 542-550 (2000).
3. Ryan, J.J. et al., Mast cell homeostasis: a fundamental aspect of allergic disease. Crit. Rev. Immunol., 27, 15-32 (2007).
4. Callard, R.E. et al., IL-4 and IL-13 receptors: are they one and the same? Immunol. Today, 17, 108-110 (1996).
5. Yokota, T. et al., Molecular biology of interleukin 4 and interleukin 5 genes and biology of their products that stimulate B cells, T cells and hemopoietic cells. Immunol. Rev., 102, 137-187 (1988).
6. Kitamura, T. et al., Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell Physiol., 140, 323-334 (1989).

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Interleukins, released from leukocytes, include IL-1β playing crucial roles in inflammation, immune responses, and anti-tumor immunity.

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