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G5035

Sigma-Aldrich

Granulocyte-Macrophage Colony-Stimulating Factor human

GM-CSF, recombinant, expressed in E. coli, suitable for cell culture

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Synonym(s):
GM-CSF human
MDL number:

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

≤0.1 ng/mL EC50

mol wt

14.6 kDa

packaging

pkg of 4X5 μg
pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1.000 EU/μg

color

white

solubility

water: soluble 0.010 mL, clear, colorless

UniProt accession no.

storage temp.

−20°C

Gene Information

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This Item
SRP3050G0282M9170
recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

form

lyophilized powder

form

lyophilized

form

lyophilized powder

form

lyophilized powder

potency

≤0.1 ng/mL EC50

potency

≤0.1 ng/mL

potency

≤0.2 ng/mL EC50 (corresponds to ≥5 × 106 units/mg)

potency

0.500-3.000 ng/mL ED50

mol wt

14.6 kDa

mol wt

14.6 kDa

mol wt

~15 kDa (125 amino acids including N-terminal methionine)

mol wt

dimer 18.2 kDa (containing 156 amino acid residues)

General description

Package size based on protein content

Biochem/physiol Actions

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

Physical form

Lyophilized from a 0.2 μm filtered, 10 mM sodium citrate, pH 3.5 solution.

Analysis Note

The proliferative activity of human GM-CSF is tested in culture using human TF-1 cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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705578-5MG-PW

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