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G0407

Sigma-Aldrich

Granulocyte colony-stimulating factor human

G-CSF, recombinant, expressed in E. coli, suitable for cell culture

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MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

0.01-0.1 ng/mL

quality

endotoxin tested

mol wt

18.8 kDa (175 amino acids including N-terminal methionine)

packaging

pkg of 5 and 25 μg

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μg (LAL test)

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... CSF3(1440)

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1 of 4

This Item
M6518G0282H8416
Stem Cell Factor human SCF, recombinant, expressed in HEK 293 cells, suitable for cell culture

H8416

Stem Cell Factor human

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE and HPLC)

assay

≥97% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

biological source

human

biological source

human

biological source

mouse

biological source

human

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

General description

Granulocyte colony-stimulating factor human (GCSF) gene is mapped to human chromosome 17q21.1. Structurally, GCSF comprises a four α-helical bundle.

Application

Granulocyte colony-stimulating factor human has been used:
  • in culturing human neutrophils
  • as a growth factor component of double layer granulocyte-macrophage colony-forming unit (CFU-GM) culture system for CD34+ cells
  • to test its protective effects chronic liver damage model

Biochem/physiol Actions

Granulocyte colony-stimulating factor human (GCSF) interacts specifically with the immunoglobin (Ig) domain of the granulocyte colony-stimulating factor receptor (GCSFR).
Granulocyte-colony stimulating factor (G-CSF) is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G0 period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.

Physical form

Lyophilized from 0.2 μm-filtered solution of 10 mM HOAc containing 50 μg BSA per μg G-CSF

Analysis Note

The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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The clonogenic potential of selected CD34+ cells from patients with MDS appear preserved when tested ex vivo
Davison GM, et al.
Leukemia Research, 35, 1200-1204 (2011)
Mohsen Esmaili et al.
Molecular biology research communications, 3(2), 141-147 (2014-06-01)
The present study aimed at investigating the beneficial effects of co-administering granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) in a model of chronic liver injury induced by thioacetamide (TAA). Biochemical and histopathology- cal examinations were performed on serum
Eijiro Honjo et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 61(Pt 8), 788-790 (2006-03-03)
The granulocyte-colony stimulating factor (GCSF) receptor receives signals for regulating the maturation, proliferation and differentiation of the precursor cells of neutrophilic granulocytes. The signalling complex composed of two GCSFs (GCSF, 19 kDa) and two GCSF receptors (GCSFR, 34 kDa) consisting
Yukinori Okada et al.
Human molecular genetics, 19(10), 2079-2085 (2010-02-23)
Neutrophils are the most abundant subtype of white blood cells (WBCs). Although the regulation of the numbers of neutrophils would have substantial clinical impacts, the studies on the variations associated with neutrophil count had not been performed further. To investigate
Paul J Hurd et al.
The Journal of biological chemistry, 284(24), 16575-16583 (2009-04-14)
Numerous post-translational modifications have been identified in histones. Most of these occur within the histone tails, but a few have been identified within the histone core sequences. Histone core post-translational modifications have the potential to directly modulate nucleosome structure and

Articles

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