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V7259

Sigma-Aldrich

Vascular Endothelial Growth Factor human

VEGF, recombinant, expressed in E. coli, powder, suitable for cell culture

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Synonym(s):
VEGF
CAS Number:
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE)

form

powder

potency

1-10 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

protein 38.2 kDa (as homodimer)

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤0.1 EU/μg

color

white

solubility

water: soluble

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... VEGFA(7422)

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This Item
V3388H9041H9166
recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

assay

≥98% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

form

powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

potency

1-10 ng/mL ED50/EC50

potency

0.75-3.75 ng/mL ED50

potency

≤15 ng/mL EC50

potency

≤5 ng/mL EC50

mol wt

protein 38.2 kDa (as homodimer)

mol wt

predicted mol wt ~28 kDa

mol wt

dimer 37 kDa (glycosylated), trimer 50 kDa (glycosylated)

mol wt

dimer 45 kDa (glycosylated)

General description

Vascular Endothelial Growth Factor (VEGF) gene is localized to human chromosome 6p21.3, and is thought to encode four different isoforms.

Application

Vascular Endothelial Growth Factor (VEGF) has been used for-
  • the determination of oligodendrocyte precursor cells (OPCs) and luteal cell proliferation/survival
  • inducing proliferation of epithelial cell clusters (ECCs) to be transplanted into mice

Biochem/physiol Actions

Vascular Endothelial Growth Factor (VEGF), also known as vasculotropin, is an angiogenic growth factor, which is heat and acid stable. VEGFs stimulate endothelial cell growth, angiogenisis, and capillary permeability. VEGF is a secreted homodimeric, heparin-binding glycoprotein,1 which has an isoelectric point of 8.5. VEGF promotes the growth of endothelial cells isolated from bovine adrenal cortex, cerebral cortex, fetal and adult aorta, and human umbilical vein. The target cell specificity of VEGF is restricted to vascular endothelial cells. VEGF has no mitogenic effect on cultured corneal endothelial cells, vascular smooth muscle cells, BHK-12 fibroblasts, keratinocytes, human sarcoma cells, or lens epithelial cells. A variety of human tumor cell lines including sarcoma and carcinoma cells show a 3.7 kb RNA transcript that hybridizes with the VEGF probe in a Northern blot. Mouse sarcoma 180 cells express the VEGF mRNA and secrete a VEGF-like mitogen.

VEGF recombinant protein might have potential as a therapeutic agent in treatment-resistant disorders, where it can facilitate endothelial cell growth and vessel formation.

Physical properties

Monomer consists of 165 amino acid residues, approx. mol. weight 19.2 kDa

Physical form

Lyophilized from a sterile filtered buffered solution with no additives.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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PloS one, 15(11), e0241442-e0241442 (2020-11-05)
Heterotopic and orthotopic ovarian tissue autotransplantation techniques, currently used in humans, will become promising alternative methods for fertility preservation in domestic and wild animals. Thus, this study describes for the first time the efficiency of a heterotopic ovarian tissue autotransplantation
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Islet transplantation offers improved glucose homeostasis in diabetic patients, but transplantation of islets is limited by the supply of donor pancreases. Undifferentiated precursors hold promise for cell therapy because they can expand before differentiation to produce a large supply of
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