SRP3287

IL-34 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Synonym(s): C16orf77
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• assay: ≥95% (HPLC); ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: 52.5 kDa
• packaging: pkg of 10 μg
• impurities: <0.1 EU/μg endotoxin, tested
• UniProt accession no.: Q6ZMJ4
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... IL34(146433)

Description

General description

IL-34 is a ligand for colony-stimulating factor-1 receptor (CSF1R), which also binds to CSF-1. It specifically binds CD14+ monocytes, promotes survival and proliferation of human peripheral blood monocytes, and stimulates macrophage colony formation by human bone marrow cells. IL-34, like human CSF-1 stimulates phosphorylation of MAPK1/ERK2 and MAPK3/ERK1.

Biochem/physiol Actions

IL-34 is a homodimeric cytokine that is expressed in a range of tissues including spleen, heart, brain, liver, kidney, lung, ovary, thymus, testis, small intestine, prostate and colon. Recombinant human IL-34 is a 52.5 kDa homodimeric glycoprotein consisting of 460 amino acid residues, including a C-terminal His-Tag.

Physical form

Lyophilized from 20 mM Sodium Citrate, pH 3.0 + 75 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319,H335
• pcodes: P261 - P264 - P271 - P280 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable