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H6916

Sigma-Aldrich

Macrophage Colony-Stimulating Factor human

M-CSF, recombinant, expressed in HEK 293 cells, suitable for cell culture

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Synonym(s):
MCSF
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤4 ng/mL EC50

quality

endotoxin tested

mol wt

dimer 35-40 kDa (glycosylated)

packaging

pkg of 10 μg

manufacturer/tradename

HumanZyme

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... CSF1(1435)

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This Item
M9170SRP3110SRP3050
Sigma-Aldrich

Sigma-Aldrich

SRP3110

M-CSF human

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

form

lyophilized powder

form

lyophilized powder

form

lyophilized

form

lyophilized

potency

≤4 ng/mL EC50

potency

0.500-3.000 ng/mL ED50

potency

≤1.0 ng/mL ED50

potency

≤0.1 ng/mL

mol wt

dimer 35-40 kDa (glycosylated)

mol wt

dimer 18.2 kDa (containing 156 amino acid residues)

mol wt

36.8 kDa

mol wt

14.6 kDa

General description

M-CSF is encoded by the gene mapped to human chromosome 1p13.3. It is a glycosylated polypeptide, which exists as a homodimer. M-CSF is produced by macrophage, fibroblasts, lymphocyte and endothelial cells.

Biochem/physiol Actions

Macrophage CSF (M-CSF) as a growth factor, stimulates the proliferation and differentiation of monocyte/macrophage-lineage cells. It also boosts antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages. In addition, human M-CSF inhibits bone resorption by osteoclasts. It might also play a crucial role in atherosclerosis. Overexpression of the gene has been observed in Paget′s disease of bone (PDB).

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (mouse myeloid Leukemia indicator cell line).

Citation

1. Mazur, E.M., and Cohen, J.L., Clin. Pharmacol. Ther., 46, 250 (1989).
2. Morstyn, G., and Burgess, A.W., Cancer Res., 48, 5624 (1988).
3. Metcalf, D., Blood, 67, 257 (1986).
4. Mufson, R.A., et al., Cell. Immunol., 119, 182 (1989).
5. Hattersley, G., et al., J. Cell Physiol., 137, 199 (1988).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Genome-wide association study identifies variants at CSF1, OPTN and TNFRSF11A as genetic risk factors for Paget's disease of bone.
Albagha O M E, et al.
Nature Genetics, 429(6), 520-520 (2010)
"Effects of macrophage colony-stimulating factor (M-CSF) on protease production from monocyte, macrophage and foam cell in vitro: a possible mechanism for anti-atherosclerotic effect of M-CSF.
Tojo N, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1452.3, 275-284 (1999)
Human macrophage colony-stimulating factor inhibits bone resorption by osteoclasts disaggregated from rat bone.
Hattersley G, et al.
Journal of Cellular Physiology, 137(1), 199-203 (1988)
Macrophage colony-stimulating factor enhances monocyte and macrophage antibody-dependent cell-mediated cytotoxicity.
Mufson R A, et al.
Cellular Immunology, 119(1), 182-192 (1989)
The molecular biology and functions of the granulocyte-macrophage colony-stimulating factors.
Metcalf D.
Blood, 67(2), 257-267 (1986)

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