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Tumor Necrosis Factor-α human

TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

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hTNF-α, TNF-α
CAS Number:
MDL number:

biological source


Quality Level


expressed in E. coli


≥97% (SDS-PAGE)




0.02-0.3 ng/mL ED50/EC50


endotoxin tested

mol wt

~17.4 kDa


pkg of 5x10 μg
pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles


cell culture | mammalian: suitable


<1 EU/μg

UniProt accession no.

storage temp.


Gene Information

human ... TNF(7124)

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This Item

expressed in E. coli


expressed in E. coli


expressed in HEK 293 cells


expressed in yeast


≥97% (SDS-PAGE)


>95% (SDS-PAGE)


≥95% (SDS-PAGE)


≥95% (SDS-PAGE)






lyophilized powder




0.02-0.3 ng/mL ED50/EC50


4-20 pg/mL Activity


≤1.0 ng/mL ED50


≤0.01 ng/mL EC50

mol wt

~17.4 kDa

mol wt

protein 18.8 kDa

mol wt

17 kDa (glycosylated), ~17.4 kDa

mol wt

17,000 Da


TNF-α include stimulating growth of human fibroblasts and other cell lines activating polymorphonuclear neutrophils and osteoclasts and inducing interleukin-1, prostaglandin E2, and collagenase production.
Tumor Necrosis Factor-α (TNFα) human has been used:
  • to analyze the effects of cytokine TNFα-stressed human neuronal and glial (HNG) cells
  • to investigate the molecular mechanisms of TNFα-mediated prolyl-4 hydroxylase α1 (P4Hα1) suppression
  • to induce death-receptor-mediated apoptosis in HeLa cells.

Biochem/physiol Actions

Tumore necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is N-glycosylated.

Physical form

Lyophilized from a 0.2 μm-filtered buffer solution containing 3mM Tris-HCl and 5% trehalose.

Analysis Note

The biological activity of TNF-α is measured by the cytolysis of murine L929 cells.

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