MilliporeSigma
All Photos(2)

11465007001

Roche

Cell Proliferation Kit I (MTT)

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
mtt

form

liquid

Quality Level

usage

sufficient for ≤2,500 tests

packaging

pkg of 1 kit

manufacturer/tradename

Roche

storage condition

protect from light

technique(s)

tissue culture: suitable

λmax

550-600 nm

application(s)

cell analysis
detection

detection method

colorimetric

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
11465015001CT01SCT110
form

liquid

form

liquid

form

-

form

-

usage

sufficient for ≤2,500 tests

usage

sufficient for ≤2,500 tests

usage

-

usage

-

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

-

packaging

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

storage condition

protect from light

storage condition

protect from light

storage condition

-

storage condition

-

General description

The Cell Proliferation Kit I (MTT) is a colorimetric assay for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity. Sample material is either adherent or suspension cells cultured in 96-well microplates.

Colorimetric assays analyze the number of viable cells by the cleavage of tetrazolium salts added to the culture medium. This technique requires neither washing nor harvesting of cells, and the complete assay, from microculture to data analysis by an ELISA reader, is performed in the same microplate.
MTT was the first tetrazolium salt described. It is cleaved to formazan by enzymes of the endoplasmic reticulum. This bioreduction occurs in viable cells only, and is related to NAD(P)H production through glycolysis. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture.

Colorimetric assay (MTT based) for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity.

Application

The Cell Proliferation Kit I (MTT) is used for the nonradioactive, spectrophotometric quantification of cell proliferation and viability in cell populations using the 96-well-plate format. It can be used for:
  • Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients
  • Analysis of cytotoxic and cytostatic compounds, such as anti-cancer drugs and other pharmaceutical compounds
  • Assessment of growth-inhibitory antibodies and physiological mediators
  • Testing of biocompatibility of various scaffolds, employed in bone tissue engineering, for bone cell growth

Features and Benefits

  • Safe and easy: Eliminate radioactive isotopes, washing steps, and additional reagents.
  • Accurate: The absorbance obtained strongly correlates to the cell number.
  • Sensitive: Detect low cell numbers.
  • Fast: Process a large number of samples using a multi-well ELISA reader.
The assay is based on the cleavage of the tetrazolium salt MTT in the presence of an electron-coupling reagent. The water-insoluble formazan salt produced must be solubilized in an additional step. Cells grown in a 96-well tissue culture plate are incubated with the MTT solution for approximately 4 hours. After this incubation period, a water-insoluble formazan dye is formed. After solubilization, the formazan dye is quantitated using a scanning multi-well spectrophotometer (ELISA reader). The measured absorbance directly correlates to the number of viable cells.
Cell proliferation and viability assays are of particular importance for routine applications in cell biology. Tetrazolium salts (e.g., MTT, XTT, WST-1) are particularly useful for this type of analysis. Tetrazolium salts are cleaved to formazan by the succinate-tetrazolium reductase system (EC 1.3.99.1) which belongs to the respiratory chain of the mitochondria, and is only active in metabolically intact cells (see below).

Packaging

1 kit containing 2 components.

Preparation Note

Storage conditions (working solution): 2 to 8 °C
After thawing, the MTT labeling reagents may be stored protected from light at 2 to 8 °C for up to 4 weeks, in which case reagent filtration through a 0.2 μm pore size membrane is recommended.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • MTT Labeling Reagent

  • Solubilization Solution

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 8

1 of 8

Naoki Tajiri et al.
CNS neuroscience & therapeutics, 22(4), 306-315 (2016-02-05)
Exportin 1 (XPO1/CRM1) plays prominent roles in the regulation of nuclear protein export. Selective inhibitors of nuclear export (SINE) are small orally bioavailable molecules that serve as drug-like inhibitors of XPO1, with potent anti-cancer properties. Traumatic brain injury (TBI) presents
Yuji Kaneko et al.
PloS one, 7(10), e47583-e47583 (2012-10-19)
This study was designed to reveal the therapeutic regimen and mechanism of action underlying hypothermia treatment in combination with stem cell transplantation for ameliorating neonatal hypoxic-ischemic-like injury. Primary rat neurons were exposed to oxygen-glucose deprivation (OGD), which produced hypoxic-ischemic-like injury
Timothy Douglas et al.
Clinical oral implants research, 21(3), 262-267 (2009-12-05)
Hydroxyapatite (HA) and tricalcium phosphate (TCP) are two very common ceramic materials for bone replacement. A recently developed material for bone replacement is CeraBall, which is a mixed HA-TCP scaffold available as porous spherical scaffolds of diameter 4 and 6
Ali Abolhassani et al.
Journal of Cancer, 5(7), 537-547 (2014-07-25)
Fibroblastic growth factor-10 (FGF-10) has an important role in type I epithelial mesenchymal transition (EMT) during the embryonic period of life (gastrulation). Since EMT has a critical role during cancer cells invasion and metastasis (type III) this study sought to
Volker Gassling et al.
Clinical oral implants research, 24(3), 320-328 (2011-11-19)
The loss of jaw bone caused by different kinds of pathologies leads to dysfunction and reduced quality of life in affected patients. Thus, the pivotal goal in bone tissue engineering is to reconstruct these defects. The essential precondition for new

Articles

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Protocols

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service