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M5655

Sigma-Aldrich

Thiazolyl Blue Tetrazolium Bromide

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

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Synonym(s):
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide
Empirical Formula (Hill Notation):
C18H16BrN5S
CAS Number:
Molecular Weight:
414.32
Beilstein/REAXYS Number:
4081397
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

product line

BioReagent

Quality Level

assay

≥97.5% (HPLC)

form

powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

mp

195 °C (dec.) (lit.)

solubility

H2O: 5 mg/mL

storage temp.

2-8°C

SMILES string

[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4

InChI

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

InChI key

AZKSAVLVSZKNRD-UHFFFAOYSA-M

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1 of 4

This Item
M212888190T4375
vibrant-m

M5655

Thiazolyl Blue Tetrazolium Bromide

vibrant-m

M2128

Thiazolyl Blue Tetrazolium Bromide

vibrant-m

88190

Tetrazolium Blue Chloride

vibrant-m

T4375

Tetrazolium Blue Chloride

solubility

H2O: 5 mg/mL

solubility

2-methoxyethanol: soluble 20 mg/mL, H2O: 5 mg/mL

solubility

methanol: 0.5 g/10 mL ( hot)

solubility

methanol: 50 mg/mL, clear

form

powder

form

powder

form

powder

form

powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

assay

≥97.5% (HPLC)

assay

98%

assay

≥90% (T)

assay

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

room temp

Application

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
Thiazolyl Blue Tetrazolium Bromide has been used as a colorimetric metabolic activity indicator in cell viability assays (MTT assay). It has also been used to determine cell proliferation.

pictograms

Exclamation markHealth hazard

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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