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Sigma-Aldrich

Cell Growth Determination Kit, MTT based

Synonym(s):

Mitochondrial activity assay

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

packaging

pkg of 1 kit

Quality Level

200

technique(s)

UV/Vis spectroscopy: suitable
protein quantification: suitable

Amax

570 nm

application(s)

cell analysis
detection

detection method

colorimetric

shipped in

dry ice

storage temp.

−20°C

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General description

Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.

Application

Cell Growth Determination Kit, MTT based has been used:
  • to measure the cell viability of preosteoblastic cells
  • in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
  • to determine the cell viability of primary cervical epithelial cells

Packaging

Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.

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Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

flash_point_f

53.6 °F - closed cup

flash_point_c

12 °C - closed cup

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