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CGD1

Sigma-Aldrich

Cell Growth Determination Kit, MTT based

Synonym(s):

Mitochondrial activity assay

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

packaging

pkg of 1 kit

technique(s)

UV/Vis spectroscopy: suitable
protein quantification: suitable

Amax

570 nm

application(s)

cell analysis
detection

detection method

colorimetric

shipped in

dry ice

storage temp.

−20°C

General description

Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.

Application

Cell Growth Determination Kit, MTT based has been used:
  • to measure the cell viability of preosteoblastic cells
  • in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
  • to determine the cell viability of primary cervical epithelial cells

Packaging

Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.

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Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

flash_point_f

53.6 °F - closed cup

flash_point_c

12 °C - closed cup


Certificates of Analysis (COA)

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Carla M R Lacerda et al.
American journal of physiology. Heart and circulatory physiology, 302(10), H1983-H1990 (2012-02-22)
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Journal of cellular and molecular medicine, 22(10), 4640-4652 (2018-07-25)
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Mengxiong Wang et al.
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While HER2 and EGFR are overexpressed in breast cancers and multiple other types of tumors, the use of EGFR and/or HER2 inhibitors have failed to cure many cancer patients, largely because cancers acquire resistance to HER2/EGFR-specific drugs. Cancers that overexpress
Ahran Pae et al.
The journal of advanced prosthodontics, 6(2), 96-102 (2014-05-21)
This study was performed to characterize the effects of zirconia coated with calcium phosphate and hydroxyapatite compared to smooth zirconia after bone marrow-derived osteoblast culture. Bone marrow-derived osteoblasts were cultured on (1) smooth zirconia, (2) zirconia coated with calcium phosphate
Mariarita Perri et al.
Biochimica et biophysica acta, 1800(9), 993-1001 (2010-07-06)
Vitamin A is suggested to be protective against oxidative stress. However, different authors observed pro-oxidant effects of retinoids both in experimental works and clinical trials. These discordances are the bases for the investigation of the proliferative and anti-proliferative properties of

Articles

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

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