96992
Cell Counting Kit - 8
for quantitation of viable cell number in proliferation and cytotoxicity assays
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About This Item
UNSPSC Code:
12352108
NACRES:
NA.32
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Quality Level
shelf life
limited shelf life, expiry date on the label
shipped in
ambient
storage temp.
2-8°C
Application
Cell Counting Kit-8 (CCK-8) allows convenient assays using WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt), which produces a water-soluble formazan dye upon bioreduction in the presence of an electron carrier, 1-Methoxy PMS. CCK-8 solution is added directly to the cells, no pre-mixing of components is required. WST-8 is bioreduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells. Since the CCK-8 solution is very stable and it has little cytotoxicity, a longer incubation, such as 24 to 48 hours, is possible.
Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS.
Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS.
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Hazard Classifications
Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
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Not applicable
flash_point_c
Not applicable
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Eyeshields, Gloves
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Articles
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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