06432379001

Cell Viability Imaging Kit 5x96

Properties

• usage: sufficient for 5 96-well plate(s)
• Quality Level: 100
• packaging: pkg of 1 kit
• manufacturer/tradename: Roche
• storage condition: protect from light
• fluorescence: λ_ex 361 nm; λ_em 486 nm (nuclei dye); λ_ex 490 nm; λ_em 515 nm (viable dye); λ_ex 535 nm; λ_em 617 nm (dead dye)
• detection method: fluorometric
• shipped in: dry ice
• storage temp.: −20°C (−15°C to −25°C)

Description

General description

The Cell Viability Imaging Kit is a simple and rapid way to measure mammalian cell viability using fluorescence microscopy and automated imaging platforms, such as the Cellavista System. Optimized for 96-well plates, the numbers of viable and dead cells, as well as total cell numbers, are determined simultaneously for the area of the well selected.

The kit is designed for 5 × 96 reactions for use in medium to high throughput analysis of cell behavior.

• Determine total cell number using fluorescence staining of cell nuclei.
• Fluorimetrically determine the percentage of dead cells based on membrane permeability.
• Verify the labeling of viable cells quantified by fluorescence staining using a vital dye

Application

Cell quantification and cell viability control are very important methods in cell biology. This includes both life science research and industrial applications. The simple, accurate and fast “one step assay” makes the Cell Viability Imaging Kit an ideal tool for quality control of cells in cellular workflows, especially for medium to high throughput applications.

Features and Benefits

Cell counting and cell viability are essential for cellular workflows. The Roche Cell Viability Imaging Kit meets the following requirements:

• No risk of losing cells in the one step assay: There are no fixation or washing steps.
• Save time and resources: Total assay time for a 1 x 96 well microplate is only 35 minutes.
• Obtain highly reproducible results using automated imaging and data evaluation with Roche′s Cellavista System.

Packaging

1 kit containing 3 components.

Preparation Note

Storage conditions (working solution): Vial 3: The product is stable until the expiration date printed on the label when stored at -15 to -25 °C. Contents can be frozen and thawed at least 5 times after preparation of solution.

Storage and Stability

Store at -15–-25 °C. (Vial 1 and Vial 2 can be frozen and thawed at least 5 times. Vial 3 can be frozen and thawed at least 5 times after preparation of solution.)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

• Nuclei Dye (blue cap) for fluorimetric determination of cell count based on labeling of cell nuclei
• Dead Dye (red cap) for fluorimetric determination of dead cells based on cell permeability
• Viable Dye ( green cap) for fluorimetric determination of viable cells based on metabolic activity

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H341
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable