P4864

Propidium iodide solution

solution (1.0 mg/ml in water)

• Synonym(s): 3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide, 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium, diiodide
• Empirical Formula (Hill Notation): C₂₇H₃₄I₂N₄
• CAS Number: 25535-16-4
• Molecular Weight: 668.39
• Beilstein: 3843838
• MDL number: MFCD00011921
• PubChem Substance ID: 24898563
• NACRES: NA.47

Properties

• Assay: ≥94%
• Quality Level: 200
• form: solution (1.0 mg/ml in water)
• solubility: water: 1 mg/mL
• εmax: 5400-6400 at 491-495 nm in phosphate buffer
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: [I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
• InChI: 1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
• InChI key: XJMOSONTPMZWPB-UHFFFAOYSA-M

Description

General description

Propidium iodide solution is used to assess neuronal death.

Application

Propidium iodide solution has been used:

• in staining DNA
• in the viability staining method to study islet viability
• to visualize F-actins and nuclei in mesenchymal stem cell sheets

Membrane impermeant, phenanthridinium intercalator dye excited by mercury or xenon-arc lamps or argon-ion laser. Suitable for use in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry. Used as a nuclear counterstain.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves