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537059

Sigma-Aldrich

Propidium Iodide

Membrane impermeable DNA intercalator.

Synonym(s):

Propidium Iodide

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About This Item

Empirical Formula (Hill Notation):
C27H34I2N4
CAS Number:
Molecular Weight:
668.39
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.84

Quality Level

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

dark red

input

sample type intact cells

fluorescence

λex ~536 nm
λem ~617 nm

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

Related Categories

General description

Extinction coefficient(493 nm): 5900 M -1cm-1;(287 nm): 55,700 M -1cm-1;
Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for flow cytometry for staining apoptotic cells and nuclei. Can be used to differentiate between apoptotic and necrotic cell death while stains only necrotic cells. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate (FITC).
Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M-1cm-1 (493 nm); 55,700 M-1cm-1 (287 nm); 13,800 M-1cm-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Membrane impermeable DNA intercalator
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Irritant (B)

Preparation Note

Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Belloc, F., et al. 1994. Cytometry 17, 59.
Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard

signalword

Warning

hcodes

Hazard Classifications

Muta. 2

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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