Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

TOX1

Sigma-Aldrich

In Vitro Toxicology Assay Kit, MTT based

Synonym(s):

mitochondrial activity assay

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352207
NACRES:
NA.84

usage

 kit sufficient for 1,000 tests

Quality Level

200

packaging

pkg of 1 kit

storage condition

dry at room temperature

λmax

570 nm

application(s)

cell analysis
detection

detection method

colorimetric

storage temp.

2-8°C

General description

This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.

Application

In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP). It has also been used to examine cell viability in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.

Biochem/physiol Actions

Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.

related product

Product No.
Description
Pricing

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1 - STOT SE 3

target_organs

Central nervous system, Respiratory system

Storage Class

3 - Flammable liquids

flash_point_f

53.6 °F

flash_point_c

12 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

J Carmichael et al.
Cancer research, 47(4), 936-942 (1987-02-15)
Drug sensitivity assays were performed using a variation of a colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)] assay on V79, CHO-AuxB1, CHRC5, NCI-H460, and NCI-H249 cell lines following optimization of experimental conditions for each cell line. Results from this assay were compared with
Dalia Chávez-García et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 108(6), 2396-2406 (2020-02-06)
Luminescent lanthanide downconversion nanoparticles (DCNPs) provide a combination of high luminescence intensity, sharp emission peaks with narrow bandwidth and a large Stokes' shift, leading to high-performance biomedical applications mainly for imaging. The purpose of this study is to present a
Anumol Shashikumar et al.
Cytotechnology, 63(5), 473-480 (2011-07-30)
This is the first report on development of a finite cell line from testicular tissues of crab, Scylla serrata. Both the explant and segregated tissues of testes yielded cells that could proliferate and grow. These cells ranged in size from
Srijayaprakash B Uppada et al.
International journal of nanomedicine, 9, 43-53 (2014-01-07)
Oligonucleotides homologous to 3'-telomere overhang (T-oligos) trigger inherent telomere-based DNA damage responses mediated by p53 and/or ATM and induce senescence or apoptosis in various cancerous cells. However, T-oligo has limited stability in vivo due to serum and intracellular nucleases. To
F Denizot et al.
Journal of immunological methods, 89(2), 271-277 (1986-05-22)
A convenient way to estimate the number of viable cells growing in microtitre tray wells is to use a colorimetric assay and an automatic microplate scanning spectrophotometer. One such assay, developed by Mosmann, depends on the reduction by living cells
View All Related Papers

Articles

Quality Control Considerations in Cell Culture

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service