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T7539

Sigma-Aldrich

Tumor Necrosis Factor-α from mouse

TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

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Synonym(s):
mTNF-α, TNF-α
MDL number:
NACRES:
NA.77

biological source

mouse

Quality Level

recombinant

expressed in E. coli

assay

≥95%

form

powder

potency

0.01-0.5 ng/mL EC50

mol wt

17 kDa

packaging

pkg of 10 μg
pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg protein endotoxin

color

white

solubility

water: soluble

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Tnf(21926)

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Tumor Necrosis Factor-α from mouse TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

T7539

Tumor Necrosis Factor-α from mouse

Tumor Necrosis Factor-α human TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

T6674

Tumor Necrosis Factor-α human

Tumor Necrosis Factor-β human TNF-β, recombinant, expressed in E. coli, powder, suitable for cell culture

T7799

Tumor Necrosis Factor-β human

Tumor Necrosis Factor-α from rat TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

T5944

Tumor Necrosis Factor-α from rat

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

biological source

mouse

biological source

human

biological source

human

biological source

rat

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

Application

Tumor Necrosis Factor-α from mouse, a potent proinflammatory cytokine has been used:
  • to activate MS1 endothelium cells
  • to induce lipolysis in adipocytes
  • to study its effect on liganded and unliganded glucocorticoid receptor (GR) in interleukin (IL)-6 and IL-8 gene regulation

Biochem/physiol Actions

Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is glycosylated.

Unit Definition

One unit is the amount required to induce half-maximal cytolysis of L929 cells.

Physical form

Lyophilized from 10 mM sodium phosphate, pH 7.5

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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