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T5944

Sigma-Aldrich

Tumor Necrosis Factor-α from rat

TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

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Synonym(s):
Cachectin, TNF-α
MDL number:
NACRES:
NA.77

biological source

rat

Quality Level

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE and HPLC)

form

powder

quality

endotoxin tested

mol wt

predicted mol wt ~17 kDa

packaging

pkg of 10 μg
pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μgtested (LAL test)

color

white

solubility

water: soluble

UniProt accession no.

storage temp.

−20°C

Gene Information

rat ... Tnf(24835)

General description

The Tnf (tumor necrosis factor) gene is mapped to rat chromosome 20p12.

Application

TNF-α influences the growth and function of both normal and neoplastic cells. TNF-α causes cytolysis or cytostasis of certain transformed cells being synergistic with γ-interferon in its cytotoxicity.
Tumor Necrosis Factor-α from rat has been used:
  • To stimulate inducible nitric oxide synthase (iNOS) expression in the macrophages as an indication of M1 macrophage activation
  • To study its effect on pancreatic β cell apoptosis
  • To evaluate the effect of progesterone on the expression of tumor necrosis factor (TNF)-α in synovial membrane
  • As a blocking antigen in the control, for immunohistochemical analysis

Biochem/physiol Actions

Tumor necrosis factor-α (TNF-α), also known as cachectin, plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication, and hematopoiesis. TNF-α is expressed by a variety of cells, with numerous inductive and suppressive agents. It is primarily produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens, and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2.

Physical form

Lyophilized from an 0.2 μm-filtered buffered solution with bovine serum albumin as a carrier.

Analysis Note

The biological activity is measured in a cytotoxic assay using a TNF-susceptible mouse L929 cell line in the presence of actinomycin D.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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