MilliporeSigma
All Photos(1)

Documents

I0406

Sigma-Aldrich

Interleukin-6 from rat

IL-6, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

Sign Into View Organizational & Contract Pricing

Synonym(s):
Interleukin-6 from rat
MDL number:

biological source

rat

Quality Level

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

form

buffered aqueous solution

potency

0.01-0.06 ng/mL ED50

quality

endotoxin tested

mol wt

predicted mol wt ~22 kDa

packaging

pkg of 10 and 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.01EU/mg (LAL test)

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

rat ... Il6(24498)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
I9646SRP3096SCU0001
vibrant-m

I0406

Interleukin-6 from rat

vibrant-m

I9646

Interleukin-6 from mouse

vibrant-m

SRP3096

Interleukin-6 human

vibrant-m

SCU0001

Interleukin-6 human

biological source

rat

biological source

mouse

biological source

human

biological source

human

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

assay

>97% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

mol wt

predicted mol wt ~22 kDa

mol wt

~21.7 kDa

mol wt

20.9 kDa

mol wt

calculated mol wt 20.8 kDa (183 amino acids glycosylated protein)

Biochem/physiol Actions

Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Physical form

Supplied as a 0.2 μm filtered solution in 50 mM sodium acetate, pH 4, with BSA as a carrier protein.

Preparation Note

Recombinant, rat Interleukin-6 is produced from a DNA sequence encoding the mature rat IL-6 protein. The methionyl form of mature rat IL-6, a 188 amino acid protein, has a predicted molecular mass of approx. 22 kDa. Rat and mouse IL-6 share 93% amino acid sequence identity.

Analysis Note

The biological activity is measured in a cell proliferation assay using IL-6 dependent mouse plasmacytoma cell line, T1165.85.2.1.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 4

1 of 4

P W Bodell et al.
Journal of applied physiology (Bethesda, Md. : 1985), 106(2), 443-453 (2008-12-06)
Childhood diseases are often accompanied by chronic inflammation, which is thought to negatively impact growth. Interleukin-6 (IL-6) is typically cited as an indicator of inflammation and is linked to impaired growth. This study was designed to isolate and identify potential
Elin Banke et al.
Metabolism: clinical and experimental, 63(6), 831-840 (2014-04-02)
The bacteria Staphylococcus aureus is part of the normal bacterial flora and produces a repertoire of enterotoxins which can cause food poisoning and toxic shock and might contribute to the pathogenesis of inflammatory diseases. These enterotoxins directly cross-link the T
Lorena M Amaral et al.
American journal of obstetrics and gynecology, 211(2), 158-158 (2014-02-20)
Preeclampsia is a multisystem disorder recognized as hypertension with proteinuria developing >20 weeks' gestation. Preeclampsia is associated with chronic immune activation characterized by increased T and B lymphocytes, cytokines, and antibodies activating the angiotensin II type I receptor (AT1-AA). Hypertension
Mingming Zhang et al.
PloS one, 9(10), e109146-e109146 (2014-10-03)
It has been shown that Faecalibacterium prausnitzii (F. prausnitzii), one of the dominant intestinal bacterial flora, may protect colonic mucosa against the development of inflammation and subsequent inflammatory bowel disease (IBD), with the underlying mechanisms being unclear. The impacts of
A O'Toole et al.
British journal of cancer, 111(5), 927-932 (2014-07-25)
Tumour microenvironment (TME) of advanced colorectal cancer (CRC) suppresses dendritic cell (DC) maturation. Here, our aim was to determine how the microenvironment of early-stage tumours influences DCs. Tumour-conditioned media (TCM) was generated by culturing explant tumour tissue in vitro (n=50).

Articles

The Interleukins comprise a disparate group of cytokines and growth factors that are produced by and released from leukocytes. Interleukin-1β (1L-1β) is released primarily from stimulated macrophages and monocytes and plays a key role in inflammatory and immune responses and may induce anti-tumor immunity.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service