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Sigma-Aldrich

Interleukin-6 human

recombinant, expressed in HEK 293 cells, suitable for cell culture

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Synonym(s):
IL-6
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

0.2-2 ng/mL EC50

quality

endotoxin tested

mol wt

calculated mol wt 20.8 kDa (183 amino acids glycosylated protein)

packaging

vial of ≥10 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤0.1 EU/μg protein (LAL test)

color

white

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... IL6(3569)

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1 of 4

This Item
I139511138600001I2644
vibrant-m

SCU0001

Interleukin-6 human

vibrant-m

I1395

Interleukin-6 human

vibrant-m

11138600001

Interleukin-6, human (hIL-6)

vibrant-m

I2644

Interleukin-2 human

biological source

human

biological source

human

biological source

human

biological source

human

Quality Level

200

Quality Level

300

Quality Level

100

Quality Level

200

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

>98% (SDS-PAGE)

assay

-

packaging

vial of ≥10 μg

packaging

pkg of 10 and 50 μg

packaging

pkg of 200,000 U (2 μg, 1 ml)

packaging

pkg of 5X10 μg, pkg of 10 μg

General description

Interleukin 6 (IL-6) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. IL-6 is a multifunctional cytokine with well-defined pro- and anti-inflammatory properties. IL-6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. The O-linked sugar chain of IL-6 is thought to contribute to the stability of the cytokine through polymerization suppression and/or conformational changes, exemplifying the significance of its expression in a human expression system (which allows proper glycosylation).

Biochem/physiol Actions

Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Physical form

This product is supplied as a lyophilized powder in phosphate buffered saline (PBS), pH 7.4, with no additives or carrier proteins. It is aseptically filled.

Preparation Note

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water to a final concentration of 100 μg/ml. Adding 0.1% recombinant human serum albumin is recommended. The product is stable for at least 2 years as supplied. After reconstitution, it is recommended to store the protein in working aliquots at –20°C.

Analysis Note

The biological activity of recombinant human IL-6 was tested in culture by measuring its ability to stimulate proliferation of the IL-6 dependent mouse T1165 cells.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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T Orita et al.
Journal of biochemistry, 115(2), 345-350 (1994-02-01)
A glycosylated form of human interleukin 6 (hIL-6) has been produced in Chinese hamster ovary (CHO) cells transfected with a cDNA clone for human IL-6. Recombinant hIL-6 was purified from a culture supernatant of the transfected CHO cells, and used
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Interleukin-6 (IL-6) is a multifunctional cytokine with well-defined pro- and anti-inflammatory properties. Although only small amounts in the picogram range can be detected in healthy humans, IL-6 expression is highly and transiently up-regulated in nearly all pathophysiological states. IL-6 induces

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