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RAB0479

Sigma-Aldrich

Rat Tumor Necrosis Factor α ELISA Kit

for serum, plasma and cell culture supernatant

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Synonym(s):
Cachectin, TNF-alpha, Tumor necrosis factor alpha

species reactivity

rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 82.3-20000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... Tnf(24835)

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RAB0480RAB0477RAB0340
Gene Information

rat ... Tnf(24835)

Gene Information

rat ... Tnf(24835)

Gene Information

mouse ... Tnf(21926)

Gene Information

human ... LTA(4049)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 82.3-20000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 82.3-20000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 60 pg/mL
standard curve range: 93.75-6000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 60 pg/mL
standard curve range: 74.07-18000 pg/mL

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

input

sample type cell lysate
sample type tissue lysate

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

input

sample type cell culture supernatant(s)
sample type plasma
sample type urine
sample type serum

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

General description

The Rat TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF-α in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Rat TNF-α

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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T1503
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25G
Pack Size/Quantity

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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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