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EZHTNFA-150K

Millipore

Human TNF-α Conferma® ELISA Kit

kit sufficient for 96 wells

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Synonym(s):
Human TNF-A ELISA, TNF-A ELISA, TNF-Alpha, Tumour Necrosis Factor Alpha ELISA

Quality Level

species reactivity

human

packaging

pkg of 1 ea

parameter

50 μL sample volume (serum or plasma)

assay range

linearity: 89-113%
(Serum, 101%)

linearity: 98-110%
(Plasma, 103%)

recovery range: 81-111%
(Plasma, 97%)

recovery range: 85-105%
(Serum,95%)

inter-assay cv: ≤7%
intra-assay cv: ≤3%
sensitivity: 1.65 pg/mL
standard curve range: 1.65-400 pg/mL

technique(s)

ELISA: suitable

input

sample type serum
sample type plasma (K2 EDTA)

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

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assay range

linearity: 89-113%
(Serum, 101%), recovery range: 81-111%
(Plasma, 97%), inter-assay cv: ≤7%
intra-assay cv: ≤3%
sensitivity: 1.65 pg/mL
standard curve range: 1.65-400 pg/mL, linearity: 98-110%
(Plasma, 103%), recovery range: 85-105%
(Serum,95%)

assay range

-

assay range

-

assay range

-

input

sample type serum
sample type plasma (K2 EDTA)

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

input

sample type cell lysate
sample type tissue lysate

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

-

detection method

colorimetric (450nm/590nm)

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

General description

TNF-α is a core biomarker for the study of inflammation, considered to be a major contributing factor to numerous diseases and pathological processes. The Conferma® ELISA for TNF-α uses highly characterized critical reagents assessed by LC-MS, RPLC-UV-MS and Surface Plasmon Resonance (SPR) to deliver ensure similarity and that the mAb / Standard relationship is maintained . Each assay is further anchored against a recombinant gold standard and a sample library to ensure strong finished kit performance lot to lot. Detailed information can be found in the Protocol & Assay Verification Report.

Research Category: Inflammation

Research Sub-Category:
Alzheimer′s Disease
Aging
Inflammation
Neurodegenerative Diseases
Metabolic Disease
Sepsis Viral Diseases
Cardiovascular Disease

Specificity

This assay was tested for cross reactivity with other human cytokines like IL-1a, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17A, CCL3, CCL4, MCP-1, TNFα, GMCSF, VEGF. There is no cross-reactivity detected with any analyte.

Application

For the detection of TNF-α by ELISA in Human Serum & Plasma.

Features and Benefits

Capture ELISA assay. Highly Verified. Suitable for 37 samples in Duplicate, with a 6 point Standard Curve, 3 Quality Controls and a Blank.

Legal Information

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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Articles

Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

Protocols

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

Related Content

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