EZMCP1

Human MCP-1 ELISA Kit

detects MCP-1 in 50 μL serum, plasma or cell culture supernatent

• Synonym(s): C-C motif chemokine 2, Monocyte chemoattractant protein 1
• UNSPSC Code: 41116158
• NACRES: NA.84

Properties

• product name: Human MCP-1 Conferma^® ELISA, quantification of human MCP-1 in serum, plasma, or culture media samples.
• clone: monoclonal
• Quality Level: 200
• species reactivity: human
• parameter: 50 μL sample volume (4hr assay)
• assay range: linearity: 94-106 (serum)%; linearity: 94-110 (plasma)%; inter-assay cv: <12.9%; intra-assay cv: <4.9%; standard curve range: 1.17-300 pg/mL; accuracy: 84-95%
• technique(s): ELISA: suitable
• input: sample type plasma (K2 EDTA); sample type serum; sample type cell culture supernatant
• UniProt accession no.: P13500
• application(s): research use
• detection method: colorimetric (450nm/590nm)
• storage temp.: 2-8°C
• Gene Information: human ... CCL2(6347)

Description

General description

Human MCP-1 is a chemokine assoscited with the attraction of Monocytes and Basophils to the site of a pathology. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. In particilar this is a marker of inflammatory disorders. It binds to chemokine receptors CCR2 and CCR4.
This is a 96 well format sandwich ELISA which uses our Conferma^® process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots.

Application

This Human MCP-1 Conferma^® ELISA kit is used for the quantification of Human MCP-1 in serum, plasma, or culture media samples. One kit is sufficient to measure 37 unknown samples in duplicate.

Research Category:

• Cytokines
• Inflammation & Immunology
• Immune Response
• Metabolic Disease

Packaging

Two boxes; each to be analyzed separately.

Analysis Note

3 hour 45 minute assay, room temperature, 1:5 dilution recommended e.g.30 μL sample + 120μL of diluent (serum, plasma, or tissue culture medium)

Legal Information

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.

Safety Information

• pictograms: GHS05,GHS07,GHS08
• signalword: Danger
• hcodes: H290 - H302 + H312 + H332 - H317 - H318 - H373 - H412
• pcodes: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Sens. 1 - STOT RE 2
• target_organs: Respiratory Tract
• Storage Class: 8A - Combustible, corrosive hazardous materials
• wgk_germany: WGK 3