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3018

Sigma-Aldrich

Transcreener® UDP2 FP Assay

Synonym(s):

Transcreener assay

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About This Item

UNSPSC Code:
41122100
NACRES:
NA.84

shipped in

dry ice

storage temp.

−20°C

General description

The Transcreener® UDP2 FP Assay is a universal biochemical HTS assay for enzymes that produce UDP, including glycosyltransferase, galactosyltransferase, glucuronyltransferase, N-acetylglucosamyltransferase, N-acetlygalactosyltransferase, xylosyltransferase, and glycogen, cellulose, lactose and hyaluronan synthases. Enzyme activity is signaled by a decrease in fluorescence polarization as the bound tracer is displaced from the Transcreener® UDP2 Antibody. The assay is a simple single step mix-and-read format enabling the use of unmodified native substrate concentrations of 1 – 1000 μM. The assay provides excellent signal at low substrate conversion, with a Z′ =0.7 and =100 millipolarization shift (mP) under normal reaction conditions.

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Quantity

3018-1K = 1,000 assay, 384-well
3018-10K = 10,000 assay, 384-well

Physical form

Kit with buffered aqueous solutions

Legal Information

Transcreener is a registered trademark of BellBrook Labs

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Traci E Battle et al.
Blood, 102(8), 3016-3024 (2003-07-12)
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Dennis S Metselaar et al.
EBioMedicine, 50, 81-92 (2019-11-19)
Pediatric high-grade gliomas (pHGG) are the leading cause of cancer-related death during childhood. Due to their diffuse growth characteristics, chemoresistance and location behind the blood-brain barrier (BBB), the prognosis of pHGG has barely improved in the past decades. As such

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