YTA 3.1.2

89040603, Reactivity Anti-L3T4 epitope B, mouse CD4 )

• UNSPSC Code: 41106514

Properties

• biological source: Rat x Rat Hybridoma
• ImmunoDon: DA rat spleen
• Myeloma: Y3Ag1.2.3
• Reactivity: Anti-L3T4 epitope B, mouse CD4
• technique(s): cell culture | mammalian: suitable
• isotype: IgG2b
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Not specified

Cell Line Description

The antibody is directed against epitope B of the L3T4 antigen. This antigen is the murine equivalent of CD4. Has been used in CD4 subset depletion in vivo and flow cytometry studies. Cambridge Enterprise Reference number AB13:www.enterprise.cam.ac.uk.

Immunogen

CBA mouse spleen cells, thymocytes

Application

Not specified

Culture Medium

Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 5% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA, trypsin or mechanical agitation maybe required to dislodge them from the plastic.

Subculture Routine

Maintain cultures between 3-9 x 10⁵ cells/ml; 5% CO₂; 37 °C. Saturation density 9 x 10⁵/ml

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.