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P4689

Sigma-Aldrich

Protein G′ from proprietary source

recombinant, expressed in E. coli, lyophilized powder

Synonym(s):

G′ protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

recombinant

expressed in E. coli

Quality Level

conjugate

unconjugated

form

lyophilized powder

capacity

~5 mg/mg, solid binding capacity (IgG)

storage temp.

−20°C

General description

Genetically engineered truncated protein G; retains affinity for IgG, but lacks albumin- and Fab- binding sites and membrane-binding regions.
Protein G is a group G Streptococcus protein and is a large multi-domain cell wall protein. It interacts with the Fc region of IgG (immunolglobulin) through its repeating 55-amino acid domain. Strain GX7809 and GX7805 contain two and three such protein repeats respectively.

Application

Protein G has been used for the analysis of mAb (monoclonal antibody) synergy towards hCG (human chorionic gonadotropin) using surface plasmon resonance (SPR), and for serum IgG galactosylation obtained from RA (rheumatoid arthrtitis) patients and MRL-lpr mice.

Biochem/physiol Actions

Protein G is implicated in the evasion of host defence response by Streptococcus, via its protein binding properties. It is interacts with α2-micorglobulin which is a predominant inhibitor of human plasma.

Physical form

Lyophilized from water.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C R Goward et al.
The Biochemical journal, 267(1), 171-177 (1990-04-01)
The gene for Protein G from Streptococcus strain G148 was cloned and expressed in Escherichia coli. The regions on the gene corresponding to the albumin-binding domains and the Fab-binding region were then deleted by site-directed mutagenesis. The translation of regions
Arie Ryvkin et al.
PloS one, 7(8), e41469-e41469 (2012-08-08)
Polyclonal serum consists of vast collections of antibodies, products of differentiated B-cells. The spectrum of antibody specificities is dynamic and varies with age, physiology, and exposure to pathological insults. The complete repertoire of antibody specificities in blood, the IgOme, is
Agalactosyl IgG and beta-1,4-galactosyltransferase gene expression in rheumatoid arthritis patients and in the arthritis-prone MRL lpr/lpr mouse.
Jeddi PA et al
Immunology, 87(4), 654-659 (1996)
Enhancement in antigen binding by a combination of synergy and antibody capture.
Klonisch T et al
Immunology, 89(2), 165-171 (1996)
Haim Ashkenazy et al.
Journal of molecular biology, 433(15), 167071-167071 (2021-05-31)
Antibodies provide a comprehensive record of the encounters with threats and insults to the immune system. The ability to examine the repertoire of antibodies in serum and discover those that best represent "discriminating features" characteristic of various clinical situations, is

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