SBVT21

SB-ratBsep-HEK293

recombinant, expressed in HEK 293 cells

• Synonym(s): Solvo membrane vesicles, Bile salt export pump, Bsep, Bsep rat membrane vesicles, Rat Abcb11
• UNSPSC Code: 12352202

Properties

• recombinant: expressed in HEK 293 cells
• form: liquid
• packaging: pkg of 500 μL
• concentration: 5 mg/mL
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: rat ... Abcb11(83569)

Description

General description

Membrane Preparations for Vesicular Transport Assays (VT) are suitable for general drug-efflux transporter interaction studies. Both substrate and inhibitor interactions can be assessed using vesicles. The success of substrate interaction studies strongly depends on the passive permeability of the compound. High permeability substrates might not be detected. Control Membranes with no, or significantly lower transporter activity are also available.

Application

In the vesicular transport assay so-called "inside-out" membrane vesicles containing ABC transporters are applied. Incubating substrates of the respective efflux transporter in the presence of the inverted membrane vesicles and ATP will allow measuring accumulation of the substrates into the vesicles. In many cases radiolabeled reporter substrates are used but recently Solvo developed the new PREDIVEZ^™ Vesicular Transport Kits that use fluorescent reporter substrates.

The standard vesicular transport assay is an inhibitory assay performed with cold test articles. This assay provides information on any interaction between the ABC transporter and the test article. The transport of the reporter substrate is measured in the presence of the test article (typically in 7 concentrations) and IC50 is defined as the concentration inhibiting the transport of the reporter substrate by 50%.

Should radiolabeled form of the investigated compound or adequate analytical methods (LC/MS, HPLC) be available, the vesicular transport assay may be performed in a direct format without the reporter substrate and may identify substrate nature of the test article. The vesicular transport substrate assay is a low throughput assay. It is suitable for low permeability test compounds as high permeability compounds may escape from the vesicles through the lipid bilayer.

Physical form

Supplied as frozen membrane vesicles, containing 5 mg/mL membrane protein, labeled with volume, catalog number (transporter) and date of production.

Legal Information

PREDIVEZ is a trademark of Solvo Biotechnology

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable