V7881

Monoclonal Anti-Vitronectin antibody produced in mouse

clone VIT-2, ascites fluid

• MDL number: MFCD00162909
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: VIT-2, monoclonal
• mol wt: antigen 65-75 kDa
• contains: 15 mM sodium azide
• species reactivity: human
• technique(s): indirect ELISA: suitable; indirect immunofluorescence: suitable using human cultured fibroblasts; microarray: suitable; western blot: 1:2,500 using a denatured and reduced preparation of purified human plasma vitronectin
• isotype: IgM
• UniProt accession no.: P04004
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... VTN(7448)

Description

General description

Monoclonal Anti-Vitronectin (mouse IgM isotype) is derived from the VIT-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified human plasma vitronectin. Vitronectin is also referred as serum-spreading factor, S-protein of complement or epibolin. This monomeric acidic protein is one of the major multifunctional cell-adhesive glycoproteins in mammalian plasma and serum. This protein is usually seen as a mixture of 75 kDa and 65 kDa polypeptides. Human plasma and serum contain 0.1-0.4 mg/ml of vitronectin which is synthesized in the liver. It is also present in amniotic fluid and urine.

Specificity

By immunoblotting, the product shows no cross-reactivity with fibronectin, laminin, merosin, collagen type IV or chondroitin sulfate types A, B and C.

Immunogen

human plasma vitronectin

Application

Monoclonal Anti-Vitronectin antibody produced in mouse has been used in:

• immunocytochemisry
• western blotting
• immunoprecipitation
• immunofluorescence
• enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Vitronectin binds to heparin, collagen, streptococci and variety of cultured cells. It also acts as an inhibitor of the complement cascade by binding to the C5b-9 complex. Vitronectin protects thrombin from inactivation by antithrombin III in the presence of heparin, binds and stabilizes the activity of plasminogen activator inhibitor and mediates many other physiological functions.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable