1.50448

SeQuant^™ ZIC^®-HILIC 200Å (3.5 µm) HPLC Columns

L × I.D. 150 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

• Synonym(s): Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
• UNSPSC Code: 41115709

Properties

• product name: SeQuant^™ ZIC-HILIC^™ 3.5µm,200Å 150 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
• material: PEEK-lined stainless steel column
• Quality Level: 100
• Agency: suitable for USP L114
• product line: SeQuant^™
• feature: endcapped: no
• parameter: 550 bar pressure; 70 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable; mass spectrometry (MS): suitable (High)
• column L × I.D.: 15 cm × 2.1 mm
• matrix: fully porous particle
• matrix active group: ZIC-HILIC bonding phase
• particle size: 3.5 μm
• pore size: 200 Å pore size
• operating pH: 2-7.5
• separation technique: hydrophilic interaction (HILIC)
• storage temp.: no temp limit

Description

General description

SeQuant^™ ZIC^®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 150 mm length x 2.1 mm ID is the optimal choice for the separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant^™ HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^™ ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^™ HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Application

• Click postsynthesis of microporous organic network@silica composites: Demonstrates the application of SeQuant ZIC-HILI columns in mixed-mode chromatography, particularly in the synthesis of composites that facilitate both reversed-phase and hydrophilic interaction chromatography. This approach is crucial for advanced pharmaceutical compound analysis and biomolecule separation technology (Sun HF et al., 2023).


• Evaluation of Hold-Up Volume Determination Methods and Markers in Hydrophilic Interaction Liquid Chromatography: Focuses on refining the precision in volume determinations using ZIC-HILIC columns, enhancing method development for pharmaceuticals and providing a basis for high-resolution mass spectrometry applications (Redon L et al., 2023).


• Determination of the polyphenolic content of berry juices: Utilizes SeQuant ZIC-HILIC technology for comprehensive two-dimensional liquid chromatography, essential for complex sample analysis in food safety and quality assessments (Arena K et al., 2023).


• Simple and sensitive determination of sulfites in Chinese herbal teas: Highlights the robustness of SeQuant ZIC-HILIC in ultra-high-performance liquid chromatography tandem mass spectrometry, ensuring the safety and compliance of herbal products with stringent health standards (Yang L et al., 2022).

Analysis Note

Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.40

Legal Information

SeQuant is a trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany