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Supelco

SeQuant ZIC®-HILIC 100Å (3.5 µm) HPLC Columns

L × I.D. 100 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

Synonym(s):

Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column

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About This Item

UNSPSC Code:
41115709

product name

SeQuant ZIC-HILIC 3.5µm,100Å 100 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC

material

PEEK-lined stainless steel column

Quality Level

Agency

suitable for USP L114

product line

SeQuant

feature

endcapped: no

parameter

550 bar pressure
70 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

10 cm × 2.1 mm

matrix

fully porous particle

matrix active group

ZIC-HILIC bonding phase

particle size

3.5 μm

pore size

100 Å pore size

operating pH

2-7.5

separation technique

hydrophilic interaction (HILIC)

storage temp.

no temp limit

General description

SeQuant ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 100 Å makes this column perfectly suitable for the separation of small polar molecules under HILIC conditions.The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant ZIC®-HILIC for analysis of melamine and related substances. SeQuant HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Legal Information

SeQuant is a trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

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Articles

This article evaluates different phase chemistries for stationary phases in Hydrophilic Interaction Liquid Chromatography (HILIC) and uses multivariate analysis for classification based on chemical modification.

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