1.52322
Chromolith® HighResolution RP-18 endcapped (monolithic) HPLC Columns
L × I.D. 100 mm × 2 mm HPLC Column
Synonym(s):
Chromolith® HighResolution RP-18 endcapped 100-2 HPLC column
About This Item
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product name
Chromolith® HighResolution RP-18 endcapped, L × I.D. 100 mm × 2 mm HPLC column
Quality Level
feature
endcapped
extent of labeling
18% Carbon loading
parameter
200 bar pressure
50 °C max. temp.
technique(s)
HPLC: suitable
mass spectrometry (MS): suitable (High)
column L × I.D.
100 mm × 2 mm
surface area
250 m2/g
matrix
highly porous
monolithic silica
matrix active group
C18 bonding phase, octadecylsilane (ODS)
pore size
1.5 μm macroporosity
15 nm mesoporosity
operating pH range
2-7.5
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General description
Chromolith® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), and the surface modification is octadecylsilane with full end-capping. Chromolith® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 2.7 to 3 μm particle size fully porous particulate column.
The column dimension of 100 mm length and 2 mm I.D. is the best choice for high-resolution separations meeting UHPLC and LC-MS needs.
Chromolith® HighResolution RP-18 endcapped columns with 2 mm internal diameter are ideal for use with UHPLC or UPLC instruments, thanks to their very small internal volumes. A particular benefit is very fast analysis, reduced sample preparation and the low column backpressure. Ultra-high performance and extremely low operating pressure make Chromolith® 2 mm I.D. columns truly unique.
Chromolith® and Chromolith® HighResolution columns are perfectly suitable for LC-MS use; however, the column dimension of 2 mm I.D. or below is preferred to be used in LC-MS methods.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® HighResolution RP-18 endcapped 5-2 guard cartridges (3 pieces) [1.52325] with the Chromolith® Guard Cartridge Holder 5 mm x 2 mm [1.52355]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
Flyer: UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
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Articles
Development of an HPLC-UV method to determine Arbutin and Hydroquinone in whitening serum using a Chromolith® HighResolution C18 100x2mm HPLC column.
A comparative UHPLC analysis of diclofenac sodium in gel using Chromolith® HighResolution RP-18 endcapped 100 x 2 mm I.D column and FPP 1.7 µm hybrid silica C18 100 x 2.1 mm I.D column with UV and MS detection.
Optimized separation of Oligo Standard 6, an internally created HPLC-UV system suitability mix, on Chromolith® RP-18e columns with an evaluation of the effect of flow rates up to 3 mL/min and ion-pairing reagent, TEAA, on the separation.
LC-UV-MS workflow details teriparatide peptide mapping, including enzymatic digestion, separation conditions, and QTOF mass spectrometer identification.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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