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HADK1MAG-61K

Millipore

MILLIPLEX® Human Adipokine Magnetic Bead Panel 1 - Endocrine Multiplex Assay

The Human Adipokine Panel 1, using the Luminex xMAP technology, enables the simultaneous analysis of five biomarkers in human serum or plasma samples.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Quality Level

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 87%
(Resistin)

accuracy: 89%
(Adiponectin)

sensitivity: 10 pg/mL
(MinDC+2SD; Adipsin)

sensitivity: 21 pg/mL
(MinDC+2SD; Adiponectin)

sensitivity: 3.5 pg/mL
(MinDC+2SD; Lipocalin-2/NGAL)

sensitivity: 4.4 pg/mL
(MinDC+2SD; Resistin)

sensitivity: 5.8 pg/mL
(MinDC+2SD; PAI-1)

standard curve range: 13-200,000 pg/mL
(Adipsin)

standard curve range: 26-400,000 pg/mL
(Adiponectin)

standard curve range: 3.2-50,000 pg/mL
(Lipocalin-2/NGAL)

standard curve range: 6.4-100,000 pg/mL
(Resistin)

standard curve range: 9.6-150,000 pg/mL
(PAI-1 (total))

inter-assay cv: <10%
intra-assay cv: <10%
(Adipsin)

inter-assay cv: <10%
intra-assay cv: <15%
(Adiponectin)

inter-assay cv: <10%
intra-assay cv: <15%
(Lipocalin-2/NGAL)

inter-assay cv: <10%
intra-assay cv: <20%
(PAI-1 (total))

inter-assay cv: <10%
intra-assay cv: <20%
(Resistin)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

The role of white adipose tissue was once thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. The Adipokine Adiponectin is involved in the regulation of lipid and glucose metabolism, influencing the body′s response to insulin. Adiponectin also has anti-inflammatory effects on the cellular lining of blood vessel walls which may help explain the association of high levels with reduced risk of heart attack.

The MILLIPLEX® Human Adipokine Panel 1 is a 5-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: Adiponectin, Adipsin, Lipocalin-2/NGAL, Total PAI-1, and Resistin. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Application

  • Analytes: Adiponectin Adipsin Lipocalin-2/NGAL PAI-1 (Total) Resistin
  • Recommended Sample Type: Human serum and plasma
  • Recommended Sample Dilution: 25 μL per well of 1:400 diluted serum or plasma
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Endocrine

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Pictograms

Skull and crossbonesEnvironment

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


Certificates of Analysis (COA)

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Emily Eruysal et al.
Alzheimer's & dementia (Amsterdam, Netherlands), 11, 646-653 (2019-09-14)
Lipocalin-2 is an acute-phase protein with pleotropic functions that has been implicated in several diseases including Alzheimer's disease (AD). However, it is unknown if circulating lipocalin-2 levels are altered in the preclinical stage of AD, where AD pathology has accumulated
Ingrid V Hagen et al.
The British journal of nutrition, 116(4), 648-657 (2016-07-02)
The aim of the present study was to examine whether high intake of lean or fatty fish (cod and farmed salmon, respectively) by healthy, normal-weight adults would affect risk factors of type 2 diabetes and CVD when compared with lean
Sulochana Wijetunge et al.
Nutrition & diabetes, 9(1), 5-5 (2019-02-20)
Mechanisms of obesity-associated insulin resistance and dysglycemia in South Asians remain relatively unknown. The objective of this study was to detect subcutaneous (SAT) vs. visceral (VAT) adipose tissue characteristics and adipocytokines associated with obesity, insulin resistance, and dysglycemia in South
Laia Bertran et al.
International journal of molecular sciences, 22(10) (2021-06-03)
The pathogenic mechanisms underlying nonalcoholic fatty liver disease (NAFLD) are beginning to be understood. RUNX1 is involved in angiogenesis, which is crucial in inflammation, but its role in nonalcoholic steatohepatitis (NASH) remains unclear. The aim of this study was to
A pilot study of active vitamin D administration and insulin resistance in African American patients undergoing chronic hemodialysis.
Hung, AM; Sundell, MB; Plotnikova, NE; Bian, A; Shintani, A; Ellis, CD; Siew, ED; Ikizler, TA
Journal of Renal Nutrition : the Official Journal of the Council on Renal Nutrition of the National Kidney Foundation null

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