HADK2MAG-61K

MILLIPLEX^® Human Adipokine Magnetic Bead Panel 2 - Endocrine Multiplex Assay

The Human Adipokine Magnetic Bead Panel 2, using the Luminex xMAP technology, enables the simultaneous analysis of nine protein biomarkers in human serum and plasma samples.

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

Properties

• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 87%; (IL-8); accuracy: 91%; (TNFα); accuracy: 94%; (Insulin); standard curve range: 0.64-10,000 pg/mL; (IL-8); standard curve range: 0.64-10,000 pg/mL; (NGF); standard curve range: 0.64-10,000 pg/mL; (TNF-α); standard curve range: 0.96-15,000 pg/mL; (IL-6); standard curve range: 1.3-20,000 pg/mL; (IL-1β); standard curve range: 1.3-20,000 pg/mL; (MCP-1); standard curve range: 38-600,000 pg/mL; (Leptin); standard curve range: 9.6-150,000 pg/mL; (HGF); standard curve range: 9.6-150,000 pg/mL; (Insulin); inter-assay cv: <15%; intra-assay cv: <10%; (IL-1β); inter-assay cv: <15%; intra-assay cv: <10%; (IL-6); inter-assay cv: <15%; intra-assay cv: <10%; (IL-8); inter-assay cv: <15%; intra-assay cv: <10%; (Insulin); inter-assay cv: <15%; intra-assay cv: <10%; (Leptin); inter-assay cv: <15%; intra-assay cv: <10%; (MCP-1); inter-assay cv: <15%; intra-assay cv: <10%; (NGF); inter-assay cv: <20%; intra-assay cv: <10%; (TNFα)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Previously the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism.

The MILLIPLEX^® Human Adipokine Panel 2 is a 9-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: HGF, IL-1β, IL-6, IL-8, Insulin, Leptin, MCP-1, NGF and TNF-α. This kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 samples in duplicate.

Panel Type: Metabolism

Specificity

Cross Reactivty
Each antibody pair in a panel is highly specific and displays insignificant cross-reactivity to other analytes in the panel.

Application

• Analytes: IL-1β, IL-6, IL-8, Insulin, Leptin, MCP-1, NGF, TNF-α
• Recommended Sample Type: Human serum and plasma
• Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma
• Assay Run Time: Overnight (16-18 hours) at 2-8°C
• Research Category: Metabolism
• Research Subcategory: Endocrine

Download the HADK2MAG-61K xPonentProtocol Template Version 3.1

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96-well plate

Everything you need in a single kit.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.

Sensitivity: See kit protocol for individual sensitives.

Legal Information

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H317,H411
• Precautionary Statements: P261 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects