17153

LS Differential Agar

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Lactobacillus Streptococcus Differential Agar
• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L ; beef extract, 5 g/L ; casein enzymic hydrolysate, 10 g/L ; L-cysteine hydrochloride, 0.3 g/L ; dextrose, 20 g/L ; papaic digest of soybean meal, 5 g/L ; sodium chloride, 5 g/L ; yeast extract, 5 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 6.1±0.2 (25 °C)
• application(s): bioburden testing; environmental; food and beverages; microbiology
• suitability: nonselective and differential for Lactobacillus spp.; nonselective and differential for Streptococcus spp.

Description

Application

For the maximum growth and differentiation of Lactobacilli and Streptococci on the basis of colonial morphology, T.T.C. reduction and casein reaction.

Preparation Note

Suspend 65.3 g in 1 litre distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and add the following sterile solutions previously kept warm at 50°C just prior to use; (1) 100 ml of 10% (w/v) aqueous solution of antibiotic free skim milk powder (Cat. No. No. 70166) sterilized at 121°C for 5 minutes.(2) 10 ml of TTC Solution (Cat. No. 17779). Mix well and pour into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves