66304

Lysine Decarboxylase Broth

NutriSelect^® Plus, suitable for microbiology

• Synonym(s): LD Broth
• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• Agency: according to ISO 10273:2017
• Quality Level: 200
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: bromo creol purple, 0.02 g/L ; dextrose, 1 g/L ; L-lysine hydrochloride, 5 g/L ; peptic digest of animal tissue, 5 g/L ; yeast extract, 3 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbe id | specific enzyme detection: suitable; microbiological culture: suitable
• final pH: 6.8±0.2 (25 °C)
• application(s): clinical testing; environmental; food and beverages; microbiology
• suitability: Enterobacter spp.; nonselective and differential for Salmonella spp.; nonselective and differential for enterobacteriaceae

Description

Application

Lysine Decarboxylase Broth is used for distinguishing the Salmonella arizonae from the Bethesda Bellerup Group of Enterobacteriaceae.

Preparation Note

Suspend 14g in 1000ml distilled water. Boil to dissolve the medium completely. Dispense in 5 ml amount into screw capped test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubed medium in an upright position. Inoculate the tubes and overlay with 2-3 ml of sterile mineral oil.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves