C2206

Anti-β-Catenin antibody produced in rabbit

whole antiserum

• Synonym(s): Anti-CTNNB, Anti-EVR7, Anti-MRD19, Anti-NEDSDV, Anti-armadillo
• MDL number: MFCD02685970
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: antigen 88-94 kDa
• contains: 15 mM sodium azide
• species reactivity: several mammalian species
• packaging: antibody small pack of 25 μL
• technique(s): dot blot: suitable using the immunogen and related peptides conjugated to BSA; immunocytochemistry: 1:2,000 using cultured MDBK cells; immunohistochemistry: 1:2,000 using bovine kidney frozen sections; western blot: 1:4,000 using cultured MDBK cells
• application(s): research pathology
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CTNNB1(1499) ; mouse ... Ctnnb1(12387) ; rat ... Ctnnb1(84353)

Description

General description

Catenin are distinct peripheral cytosolic proteins and are classified into α-, β- and γ-catenin (102 kDa, 94 kDa and 86 kDa respectively. It is found in varying abundance in many developing and adult tissues.

Specificity

The antibody does not cross react with α-catenin or γ-catenin (plakoglobin).

Immunogen

synthetic peptide corresponding to the C-terminal region (amino acids 768-781) of human/mouse β-catenin, conjugated with gluteraldehyde to KLH.

Application

Anti-β-Catenin antibody produced in rabbit has been used in

• co-immunoprecipitation
• immunoblotting
• immunofluorescence
• immunoperoxidase assay

Biochem/physiol Actions

Catenins bind, directly or indirectly, to the conserved cytoplasmic tail domain of the cell adhesion cadherins. The protein, β-catenin, binds directly to the cytoplasmic tail of E-cadherin. Plakoglobin (probably identical to γ-catenin) and β-catenin are structural and possibly functional mammalian homologues of armadillo (arm), a Drosophila protein involved in signal transduction. It binds to the amino terminus of α-catenin and also interacts with the cytosolic protein product of the human tumor suppressor gene Adenomatous polyposis coli (APC). The central core region of β-catenin is involved in mediation of the interaction of cadherin-catenin complex with the epidermal growth factor receptor. The protein, β-catenin, is the target of two signal transduction pathways mediated by the protooncogenes Src and wingless-related integration site (Wnt-1). p120cas which exhibits structural similarity to β-catenin and plakoglobin may represent another catenin associated with cadherin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable