C4231

Anti-phospho-β-Catenin (pSer³³/pSer³⁷) antibody, Mouse monoclonal

clone BC-22, purified from hybridoma cell culture

• Synonym(s): Anti-CTNNB, Anti-EVR7, Anti-MRD19, Anti-NEDSDV, Anti-armadillo
• MDL number: MFCD03457566
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: BC-22, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~94 kDa
• species reactivity: mouse, human, rat, chicken
• concentration: ~2 mg/mL
• technique(s): indirect ELISA: suitable; microarray: suitable; western blot: 5-10 μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132
• isotype: IgG2b
• UniProt accession no.: P35222
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer33/pSer37)
• Gene Information: human ... CTNNB1(1499) ; mouse ... Ctnnb1(12387) ; rat ... Ctnnb1(84353)

Description

General description

CTNNB1 (catenin β 1) gene is mapped to human chromosome 3p21. The encoded protein β-catenin is localized to the nucleus.

Specificity

The antibody is inhibited more efficiently by the pSer³⁷ peptide than by the pSer³³ peptide and is not inhibited by the nonphos­phorylated peptide. It does not detect the unphos­phorylated or the Ser³³ phosphorylated protein and does not recognize phosphorylated plakoglobin.

Immunogen

synthetic phosphopeptide corresponding to the region that contains serines 33 and 37 of human β-catenin.

Application

Monoclonal Anti-phospho-ß-Catenin (pSer33/pSer37) antibody is suitable for:

• indirect ELISA
• microarray
• western blot at a dilution of 5-10μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132.

Biochem/physiol Actions

β-catenin is mapped on the short arm of chromosome 3. It is useful for differential diagnosis of Osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. It may serve as a prognostic marker for CCA progression and hence can be significant in the CCA therapy target.
In Wnt/β-catenin signal pathway β-Catenin participates as a signal transduction molecule. The pathway is associated with development, tissue homeostasis and oncogenic transcription. Upregulation of the gene is associated with glioma cell proliferation. The protein encoded has dual function that regulates the coordination of cell-cell adhesion by linking cytoskeleton and cellular junctions and gene transcription. Wnt/β-catenin signal pathway is known to induce progression colon cancer progression.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable