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NUC101

Sigma-Aldrich

Nuclei Isolation Kit: Nuclei EZ Prep

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

Synonym(s):

Rapid mammalian nuclei isolation kit

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1 KIT
$329.00

$329.00

Estimated to ship onMay 28, 2025

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1 KIT
$329.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.32

$329.00

Estimated to ship onMay 28, 2025

usage

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

shelf life

1 yr at 2‑8 °C

packaging

pkg of 1 kit

application(s)

cell analysis

foreign activity

nuclease and protease, free

shipped in

wet ice

storage temp.

2-8°C

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This Item
NUC201NXTRACT17-375
Nuclei Isolation Kit: Nuclei EZ Prep sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

NUC101

Nuclei Isolation Kit: Nuclei EZ Prep

Nuclei Isolation Kit: Nuclei PURE Prep sufficient for 15 nuclei preparations (~1-10×107 cells or 1g of tissue per preparation)

NUC201

Nuclei Isolation Kit: Nuclei PURE Prep

NuCLEAR™ Extraction Kit For mammalian tissue or cultured cells

NXTRACT

NuCLEAR™ Extraction Kit

EZ-Zyme™ Chromatin Prep Kit Contains proprietary reagents optimized for the enzymatic shearing of chromatin from mammalian cells at higher resolution than sonication for use in chromatin immunoprecipitation (ChIP) assays.

17-375

EZ-Zyme™ Chromatin Prep Kit

shelf life

1 yr at 2‑8 °C

shelf life

-

shelf life

-

shelf life

-

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

-

packaging

-

application(s)

cell analysis

application(s)

cell analysis

application(s)

-

application(s)

sample preparation

foreign activity

nuclease and protease, free

foreign activity

nuclease and protease, free

foreign activity

-

foreign activity

-

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

General description

The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.

Application

Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.

Biochem/physiol Actions

The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

Other Notes

All procedures should be carried out on ice or 2-8°C.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000384988
    0000313662
    0000333645
    0000384988
    0000320648

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    Analysis of nuclear RNA, Chapter 14.
    Robert E. Farrell, Jr., ed.
    RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)
    Christina N Vallianatos et al.
    Communications biology, 3(1), 278-278 (2020-06-03)
    Histone H3 lysine 4 methylation (H3K4me) is extensively regulated by numerous writer and eraser enzymes in mammals. Nine H3K4me enzymes are associated with neurodevelopmental disorders to date, indicating their important roles in the brain. However, interplay among H3K4me enzymes during
    Yu-Yin Shih et al.
    PloS one, 6(10), e26236-e26236 (2011-10-25)
    Retinoic acid (RA) has been approved for the differentiation therapy of neuroblastoma (NB). Previous work revealed a correlation between glucose-regulated protein 75 (GRP75) and the RA-elicited neuronal differentiation of NB cells. The present study further demonstrated that GRP75 translocates into
    Srikanth Pendyala et al.
    Antioxidants & redox signaling, 11(4), 747-764 (2008-09-12)
    In vascular endothelium, the major research focus has been on reactive oxygen species (ROS) derived from Nox2. The role of Nox4 in endothelial signal transduction, ROS production, and cytoskeletal reorganization is not well defined. In this study, we show that
    Baochen Shi et al.
    BMC genomics, 10, 92-92 (2009-02-27)
    A major goal of post-genomics research is the integrated analysis of genes, regulatory elements and the chromatin architecture on a genome-wide scale. Mapping DNase I hypersensitive sites within the nuclear chromatin is a powerful and well-established method of identifying regulatory
    View All Related Papers

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