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FCMAB317PE

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Milli-Mark® Anti-NeuN-PE Antibody, clone A60

clone A60, Milli-Mark®, from mouse

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Synonym(s):
Neuron-Specific Nuclear Protein, Neuna60, A60
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

PE

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

A60, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RBFOX3(146713)

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MAB377BMAB377MAB377C3
Anti-NeuN Antibody, clone A60 clone A60, Chemicon®, from mouse

MAB377

Anti-NeuN Antibody, clone A60

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

Gene Information

human ... RBFOX3(146713)

Gene Information

human ... RBFOX3(146713)
mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Gene Information

human ... RBFOX3(146713)
mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Gene Information

mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

conjugate

PE

conjugate

biotin conjugate

conjugate

-

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

species reactivity

human

species reactivity

rat, mouse

species reactivity

avian, pig, chicken, human, rat, salamander, ferret, mouse

species reactivity

rat

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General description

NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5.

Specificity

Antibody recognizes Neuronal Nuclei.

Immunogen

Purified cell nuclei from mouse brain.

Application

Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
This Milli-Mark Anti-NeuN-PE Antibody, clone A60 is validated for use in FC for the detection of NeuN.

Quality

Evaluated by flow cytometry using U251 cells.

Physical form

Protein A purified
Purified mouse monoclonal IgG1 conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
U251 cells

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Jeffrey Gu et al.
Frontiers in neuroscience, 15, 652226-652226 (2021-05-18)
Parkinson's disease (PD) and dementia with Lewy body (DLB) are the most common synucleinopathies. SNCA gene is a major genetic risk factor for these diseases group, and dysregulation of its expression has been implicated in the genetic etiologies of several
Federico Abascal et al.
Nature, 593(7859), 405-410 (2021-04-30)
Somatic mutations drive the development of cancer and may contribute to ageing and other diseases1,2. Despite their importance, the difficulty of detecting mutations that are only present in single cells or small clones has limited our knowledge of somatic mutagenesis
Kathryn Vaillancourt et al.
Molecular psychiatry, 26(7), 3134-3151 (2020-10-14)
Epigenetic mechanisms, like those involving DNA methylation, are thought to mediate the relationship between chronic cocaine dependence and molecular changes in addiction-related neurocircuitry, but have been understudied in human brain. We initially used reduced representation bisulfite sequencing (RRBS) to generate
A Vanessa Stempel et al.
Neuron, 90(4), 795-809 (2016-05-03)
Endocannabinoids (eCBs) exert major control over neuronal activity by activating cannabinoid receptors (CBRs). The functionality of the eCB system is primarily ascribed to the well-documented retrograde activation of presynaptic CB1Rs. We find that action potential-driven eCB release leads to a
Kathryn Vaillancourt et al.
iScience, 24(10), 103169-103169 (2021-10-26)
Cocaine dependence is a chronic, relapsing disorder caused by lasting changes in the brain. Animal studies have identified cocaine-related alterations in striatal DNA methylation; however, it is unclear how methylation is related to cocaine dependence in humans. We generated methylomic

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