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MAB377X

Sigma-Aldrich

Anti-NeuN Antibody, clone A60, Alexa Fluor488 conjugated

clone A60, Chemicon®, from mouse

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Synonym(s):
Neuron-Specific Nuclear Protein
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

ALEXA FLUOR 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

A60, monoclonal

species reactivity

human, rat, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

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This Item
MAB377BMAB377ABN91
Anti-NeuN Antibody, clone A60 clone A60, Chemicon®, from mouse

MAB377

Anti-NeuN Antibody, clone A60

Anti-NeuN Antibody from chicken, purified by affinity chromatography

ABN91

Anti-NeuN Antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

Gene Information

human ... RBFOX3(146713)
mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Gene Information

human ... RBFOX3(146713)
mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Gene Information

human ... RBFOX3(146713)
mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Gene Information

mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

species reactivity

human, rat, mouse

species reactivity

rat, mouse

species reactivity

avian, pig, chicken, human, rat, salamander, ferret, mouse

species reactivity

mouse, rat

clone

A60, monoclonal

clone

A60, monoclonal

clone

A60, monoclonal

clone

polyclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

chicken

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General description

NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).

Specificity

Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei). MAB377X reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. The immunohistochemical staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types not reactive with MAB377X include Purkinje, mitral and photoreceptor cells.

Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones.

The antibody is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants.

Immunogen

Purified cell nuclei from mouse brain.

Application

Anti-NeuN Antibody, clone A60, Alexa Fluor488 conjugated is an antibody against NeuN for use in IH.
Immunohistochemistry: 1:100 on rat (paraformaldehyde fixed) and mouse (paraformaldehyde fixed, antigen retrieved) brain tissue.



Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Physical form

Protein A purified
Purified immunoglobulin conjugated to Alexa Fluor 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide.

Storage and Stability

Maintain for 6 months at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Brain tissue, most neuronal cell types throughout the adult nervous system

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Alexa Fluor is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Gongliang Zhang et al.
Neuropharmacology, 102, 295-303 (2015-12-15)
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