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MABN140

Sigma-Aldrich

Anti-NeuN (rabbit monoclonal) Antibody, clone 27-4

clone 27-4, from rabbit

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eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

27-4, monoclonal

species reactivity

rat, mouse

species reactivity (predicted by homology)

human (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RBFOX3(146713)
mouse ... Rbfox3(52897)

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This Item
ABN91SAB4300883MAB377B
Anti-NeuN Antibody from chicken, purified by affinity chromatography

ABN91

Anti-NeuN Antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

Gene Information

human ... RBFOX3(146713)
mouse ... Rbfox3(52897)

Gene Information

mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Gene Information

human ... RBFOX3(146713)

Gene Information

human ... RBFOX3(146713)
mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

biological source

rabbit

biological source

chicken

biological source

rabbit

biological source

mouse

species reactivity

rat, mouse

species reactivity

mouse, rat

species reactivity

human, mouse, rat

species reactivity

rat, mouse

Quality Level

100

Quality Level

100, 200

Quality Level

100

Quality Level

100

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General description

NeuN is a RNA-binding protein that belongs to the evolutionarily conserved Fox-1 family. It is mainly involved RNA splicing. It is found exclusively in the nuclei of neuronal cells, but is also present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although. Some neurons fail to recognize NeuN at all ages in certain cells. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Immunogen

Recombinant protein corresponding to mouse NeuN.

Application

Anti-NeuN (rabbit monoclonal) Antibody, clone 27-4 detects level of NeuN (rabbit monoclonal) & has been published & validated for use in ICC, Western Blotting & IHC.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 cortex and hippocampus primary cultures.

Immunohistochemistry Analysis: A 1:400 dilution of this antibody detected NeuN in mouse cerebellum and hippocampus tissues.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blot in mouse brain nuclear extract.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected NeuN in 10 µg of mouse brain nuclear extract.

Target description

~46/48 kDa observed

Physical form

Format: Purified
Protein A purified
Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain nuclear extract

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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