MilliporeSigma
All Photos(4)

Documents

ABN90

Sigma-Aldrich

Anti-NeuN Antibody

serum, from guinea pig

Sign Into View Organizational & Contract Pricing

eCl@ss:
32160702
NACRES:
NA.41

biological source

guinea pig

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, mouse

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
ABN91ABN78C3MAB377C3
vibrant-m

ABN90

Anti-NeuN Antibody

vibrant-m

ABN91

Anti-NeuN Antibody

Quality Level

100

Quality Level

100, 200

Quality Level

100

Quality Level

100

antibody form

serum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

Gene Information

mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Gene Information

mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Gene Information

mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Gene Information

mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

A60, monoclonal

technique(s)

immunocytochemistry: suitable, western blot: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunocytochemistry: suitable

technique(s)

immunocytochemistry: suitable

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

ABN90 is a Guinea Pig polyclonal version of the Anti-NeuN, clone A60 (MAB377), a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are some examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Application

  • Immunohistochemistry (Paraffin) Analysis: A 1:5,000 dilution from a representative lot detected NeuN in mouse cerebral cortex and hippocampus brain tissue sections.
  • Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in rat E18 cortical cells.
  • Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
This guinea pig polyclonal Anti-NeuN, Cat. No. ABN90 is tested for use in Western Blotting, Immunocytochemistry and Immunohistochemistry (Paraffin), for the detection of NeuN.

Quality

Evaluated by Western Blotting in Mouse E16 brain tissue lysate. Western Blotting Analysis: A 1:2,000 Dilution of this antibody detected NeuN in Mouse E16 brain tissue lysate.

Target description

~48 kDa observed

Physical form

Guinea pig polyclonal serum containing 0.05% sodium azide.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Haring J Nauta et al.
Cureus, 10(3), e2371-e2371 (2018-05-29)
Punctate midline myelotomy (PMM) has been successfully applied clinically in humans for the relief of intractable visceral pain. The operation is thought to work by interrupting the postsynaptic dorsal column pathway (PSDC) of the spinal cord. In fact, PMM was developed specifically
Christopher Sliwinski et al.
Journal of neurotrauma, 35(18), 2222-2238 (2018-05-01)
A large proportion of patients suffering from spinal cord injury (SCI) develop chronic central neuropathic pain. Previously, we and others have shown that sensorimotor training early after SCI can prevent the development of mechanical allodynia. To determine whether training initiated
Baptiste Lacoste et al.
Neuron, 83(5), 1117-1130 (2014-08-27)
Neurovascular interactions are essential for proper brain function. While the effect of neural activity on cerebral blood flow has been extensively studied, whether or not neural activity influences vascular patterning remains elusive. Here, we demonstrate that neural activity promotes the
Tadasuke Tominaga et al.
PloS one, 14(3), e0213673-e0213673 (2019-03-12)
Primary and secondary traumatic brain injury (TBI) can cause tissue damage by inducing cell death pathways including apoptosis, necroptosis, and autophagy. However, similar pathways can also lead to senescence. Senescent cells secrete senescence-associated secretory phenotype proteins following persistent DNA damage
Kathryn H Fife et al.
eLife, 6 (2017-07-26)
Stopping or pausing in response to threats, conflicting information, or surprise is fundamental to behavior. Evidence across species has shown that the subthalamic nucleus (STN) is activated by scenarios involving stopping or pausing, yet evidence that the STN causally implements

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service