MilliporeSigma
All Photos(4)

Documents

N4142

Sigma-Aldrich

Anti-Neurofilament 200 antibody produced in rabbit

enhanced validation

IgG fraction of antiserum, buffered aqueous solution

Sign Into View Organizational & Contract Pricing

Synonym(s):
NF200 Antibody - Anti-Neurofilament 200 antibody produced in rabbit, Nf200 Antibody, AntiNeurofilament heavy chain
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 200 kDa

species reactivity

bovine, wide range

packaging

antibody small pack of 25 μL

enhanced validation

independent ( Antibodies)
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using animal cerebellum sections
microarray: suitable
western blot: suitable

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
N5389N0142SAB4200747
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

antibody form

IgG fraction of antiserum

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

clone

polyclonal

clone

NE14, monoclonal

clone

N52, monoclonal

clone

NE14, monoclonal

form

buffered aqueous solution

form

-

form

-

form

buffered aqueous solution

mol wt

antigen 200 kDa

mol wt

antigen apparent mol wt 200 kDa

mol wt

-

mol wt

~200 kDa

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Neurofilament 200, also known as neurofilament heavy polypeptide (NEFH), is encoded by the gene mapped to human chromosome 22q12.2. The encoded protein has a molecular mass of 200kDa and belongs to the group of type IV intermediate filaments which are key constituents of the neuronal cytoskeleton. NEFH contains C-terminal motifs involved in the formation of sidearm projections which cross-link the neurofilaments.

Specificity

Shows a wide species cross-reactivity. Reacts with neurofilaments in the central and peripheral nervous system and localizes the neurofilament polypeptide.

Immunogen

Neurofilament 200 from bovine spinal cord.

Application

Anti-neurofilament 200 antibody produced in rabbit has been used for immunolabeling.
Anti-neurofilament 200 antibody produced in rabbit has been used:
  • for detection of neurofilaments using immunohistological staining
  • in human astrocytoma cell line
  • in post-mortem brain sections
  • in human cremaster muscles
  • for detection of neurofilaments by immunofluorescence staining in lumbar spinal cord sections of mice

Biochem/physiol Actions

Neurofilament 200 plays a vital role in cytoarchitecture organization. Downregulated expression of the gene has been observed in schizophrenia patients. Loss of gene expression is associated with the development of prostate carcinoma. Mutation in the gene leads to sporadic amyotrophic lateral sclerosis (ALS).
Neurofilament 200 assembly during development is critical for normal functioning of neuronal cells. Mutations in gene encoding neurofilaments is associated with neurological disorder called the Charcot–Marie–Tooth.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at -20°C. For continuous use, the product may be stored at 2-8°C for up to one month. For extended storage, solution may be frozen in working aliquots at –20°C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Peripherin-mediated death of motor neurons rescued by overexpression of neurofilament NF-H proteins.
Beaulieu JM and Julien JP
Journal of Neurochemistry, 85(1), 248-256 (2003)
Control of neurulation by the nucleosome assembly protein-1-like 2.
Rogner UC, et al.
Nature Genetics, 25, 431-435 (2000)
Hydrogen Peroxide-Induced Neurotoxicity in Cultured Cortical Cells Grown in SerumFree
and Serum-Containing Media
Ricart KC and Fiszman ML.
Neurochemical Research, 26, 801-808 (2001)
In vivo magnetic resonance imaging of ferritin-based reporter visualizes native neuroblast migration
Iordanova B and Ahrens ET.
Neuroimage, 59, 1004-1012 (2012)
Expression pattern of NOGO-A protein in the human nervous system.
Buss A, et al.
Acta Neuropathologica, 110(2), 113-119 (2005)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service