MilliporeSigma
All Photos(1)

Documents

NE1022

Sigma-Aldrich

PhosphoDetect Anti-Neurofilament H Mouse mAb (SMI-31)

liquid, clone SMI-31, Calbiochem®

Sign Into View Organizational & Contract Pricing

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

SMI-31, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

chicken, Xenopus

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... NEFH(4744)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
NE1019OP143525295
Anti-MDM2 (Ab-3) Mouse mAb (4B11) liquid, clone 4B11, Calbiochem®

OP143

Anti-MDM2 (Ab-3) Mouse mAb (4B11)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

Gene Information

human ... NEFH(4744)

Gene Information

human ... MBP(4155)

Gene Information

human ... MDM2(4193)
mouse ... Mdm2(17246)

Gene Information

human ... PID1(55022)

species reactivity

chicken, Xenopus

species reactivity

guinea pig (weakly), mammals

species reactivity

mouse, human

species reactivity

-

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Mouse monoclonal antibody supplied as purified antibody. Recognizes the ~180-200 kDa phosphorylated neurofilament H protein.
Recognizes the ~180-200 kDa phosphorylated neurofilament H protein in rat central nervous system (CNS) cytoskeletal preparations. Also weakly recognizes phosphorylated NF-M protein.
This PhosphoDetect Anti-Neurofilament H Mouse mAb (SMI-31) is validated for use in ELISA, Frozen Sections, Immunoblotting, ICC, Paraffin Sections, IP for the detection of Neurofilament H.

Immunogen

homogenized, hypothalami from Fischer 344 rat brain

Application


ELISA (1:1000)
Frozen Sections (1:1000, see comments)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000, see comments)
Paraffin Sections (1:1000, heat pre-treatment required, see comments)
Immunoprecipitation (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS and Thimerosal

Other Notes

Raina, A.K., et al. 1999. Neuroreport10, 1355.
Yang, C.C., et al. 1998. Brain121, 1089.
Giasson, B.I and Mushynski, W.E. 1996. J. Biol. Chem.271, 30404.
Mirabella, M., et al. 1996. J. Neuropath. Exp. Neurol.55, 774.
Xiao, J. and Monteiro, M.J. 1994. J. Neurosci.14, 1820.
Strongly recognizes phosphorylated neurofilament H and, to a lesser extent, phosphorylated neurofilament M. By immunocytochemistry this antibody broadly stains thick and thin axons and some dendrites, such as basket cell dendrites, but not Purkinje cell dendrites. Does not generally stain nerve cell bodies or other cells and tissues except peripheral axons. May also stain neuronal cell bodies in pathological conditions. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures following treatment with agents that induce stress-activated protein kinase. This antibody is reported to co-immunoprecipitate neurofilament-associated kinase (NAK 115) via interaction of the antibody with the tail domain of neurofilament H. Phosphatase treatment of tissue sections or immunoblotting samples abolishes antibody reactivity. Reactivity is unaffected by trypsin treatment of samples. Tissues and cultured cells can be fixed with a variety of paraformaldehyde- or formaldehyde-containing fixatives, including Bouin′s fixative. Post-fixation in cold methanol or methanol/hydrogen peroxide facilitates access of the antibody to the epitope in frozen sections or thick tissue sections fixed in 4% paraformaldehyde and in cultured cells. For staining formalin-fixed, paraffin sections it is recommended that the de-paraffinized tissue be autoclaved in dH2O for 10 min or boiled in Tris-buffered saline, pH 9.0, in a microwave for 15 min to expose the epitope. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Takuya Hayashi et al.
Molecular neurodegeneration, 8, 4-4 (2013-01-22)
Neurodegenerative diseases including Parkinson's and Alzheimer's diseases progress slowly and steadily over years or decades. They show significant between-subject variation in progress and clinical symptoms, which makes it difficult to predict the course of long-term disease progression with or without
Seungyong Lee et al.
Nature communications, 12(1), 4939-4939 (2021-08-18)
Pain is a central feature of soft tissue trauma, which under certain contexts, results in aberrant osteochondral differentiation of tissue-specific stem cells. Here, the role of sensory nerve fibers in this abnormal cell fate decision is investigated using a severe
Justin C Burrell et al.
Neurosurgery, 87(4), 833-846 (2020-05-12)
Millions of Americans experience residual deficits from traumatic peripheral nerve injury (PNI). Despite advancements in surgical technique, repair typically results in poor functional outcomes due to prolonged periods of denervation resulting from long regenerative distances coupled with slow rates of
Michael J Whitehead et al.
Scientific reports, 8(1), 5219-5219 (2018-03-28)
Axon degeneration underlies many nervous system diseases; therefore understanding the regulatory signalling pathways is fundamental to identifying potential therapeutics. Previously, we demonstrated heparan sulphates (HS) as a potentially new target for promoting CNS repair. HS modulate cell signalling by both
Chongbo Zhong et al.
Frontiers in neural circuits, 16, 978837-978837 (2022-10-11)
Modulation of the release of glutamate by activation of presynaptic nicotinic acetylcholine receptors (nAChRs) is one of the most prevalent mechanism of nicotinic facilitation of glutamatergic transmission in cortico-limbic circuits. By imaging gene chimeric co-cultures from mouse, we examined the

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service