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AB5539

Sigma-Aldrich

Anti-Neurofilament H Antibody

Chemicon®, from chicken

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Synonym(s):
NF-H
eCl@ss:
32160702
NACRES:
NA.41

biological source

chicken

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, pig, bovine, feline, human, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

bovine ... Nefh(528842)
cat ... Nefh(101087272)
human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)

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This Item
SAB4200747N0142MAB5446
vibrant-m

AB5539

Anti-Neurofilament H Antibody

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

biological source

chicken

biological source

mouse

biological source

mouse

biological source

mouse

NCBI accession no.

NM_021076.2

NCBI accession no.

-

NCBI accession no.

-

NCBI accession no.

NM_021076.2

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

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Specificity

Recognizes Neurofilament-Heavy (NF-H). The antibody stains sharply defined cytoplasmic filaments in neuronal cell bodies and especially axons.

Immunogen

Purified bovine NF-H.

Application

Anti-Neurofilament H Antibody is an antibody against Neurofilament for use in IC, IH, IH(P) & WB.
Western blot: 1:1,000-1:10,000 using ECL. Recognizes a band of 200-220 kDa.

Immunohistochemistry: 1:1,000-1:10,000 on frozen or paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde.

Immunocytochemistry: 1:1,000-1:10,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.

Optimal working dilutions must be determined by the end user.

Target description

200 kDa

Physical form

Format: Purified
Purified chicken IgY in PBS containing 5mM sodium azide.

Analysis Note

Control
Positive control: brain.

Negative control: kidney, liver.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Computation identifies structural features that govern neuronal firing properties in slowly adapting touch receptors.
Lesniak, DR; Marshall, KL; Wellnitz, SA; Jenkins, BA; Baba, Y; Rasband, MN; Gerling, GJ; Lumpkin, EA
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Zu-Lin Chen et al.
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Laminins have dramatic and varied actions on neurons in vitro. However, their in vivo function in brain development is not clear. Here we show that knockout of laminin gamma1 in the cerebral cortex leads to defects in neuritogenesis and neuronal
Lucas M Viana et al.
Hearing research, 327, 78-88 (2015-05-24)
Recent animal work has suggested that cochlear synapses are more vulnerable than hair cells in both noise-induced and age-related hearing loss. This synaptopathy is invisible in conventional histopathological analysis, because cochlear nerve cell bodies in the spiral ganglion survive for
Katarzyna Dover et al.
Nature communications, 7, 12895-12895 (2016-09-27)
Neurons in vertebrate central nervous systems initiate and conduct sodium action potentials in distinct subcellular compartments that differ architecturally and electrically. Here, we report several unanticipated passive and active properties of the cerebellar granule cell's unmyelinated axon. Whereas spike initiation
Juan Miguel Jimenez-Andrade et al.
Bone, 46(2), 306-313 (2009-09-22)
Although musculoskeletal pain is one of the most common causes of chronic pain and physical disability in both developing and developed countries, relatively little is known about the nerve fibers and mechanisms that drive skeletal pain. Small diameter sensory nerve

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