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Neural Stem Cell Basal Medium

The Neural Stem Cell Basal Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth & in vitro differentiation of neural stem cells derived from rodents.

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Synonym(s):
NSC Culture Media
eCl@ss:
32160801

Quality Level

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type neural stem cell(s)

shipped in

dry ice

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S3194SCM005SCM004
technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

Quality Level

300

Quality Level

-

Quality Level

100

Quality Level

100

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

input

sample type neural stem cell(s)

input

-

input

sample type neural stem cell(s)

input

sample type neural stem cell(s)

General description

The Neural Stem Cell Basal Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of neural stem cells derived from rodents. When used in conjunction with bFGF or bFGF, EGF and heparin, the basal medium will allow for the proliferation of rat and mouse neural stem cells, respectively. Withdrawal of the growth factors from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of rodent neural stem cells.

Application

The Neural Stem Cell Basal Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth & in vitro differentiation of neural stem cells derived from rodents.

Quality

Each lot of medium is tested for the optimal cell growth, proliferation and differentiation of both rat and mouse neural stem cells. Sterility Testing: Negative; Osmolarity: 299; pH: 7.4

Storage and Stability

Store at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Jase Gehring et al.
Nature biotechnology, 38(1), 35-38 (2019-12-25)
We describe a universal sample multiplexing method for single-cell RNA sequencing in which fixed cells are chemically labeled by attaching identifying DNA oligonucleotides to cellular proteins. Analysis of a 96-plex perturbation experiment revealed changes in cell population structure and transcriptional

Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

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