Skip to Content
MilliporeSigma
All Photos(1)

Documents

SCM004

Sigma-Aldrich

ENStem-A Neural Expansion Medium

The ENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture & expansion of ENStem-A Human Neural Progenitor Cells.

Sign Into View Organizational & Contract Pricing

Synonym(s):
Neural Stem Cell Media
UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.71

Quality Level

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type neural stem cell(s)

shipped in

dry ice

General description

ENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture and expansion of ENStem-A Human Neural Progenitor Cells. When used in conjunction with L-Glutamine (not provided) and FGF-2 (provided), the expansion medium will allow for the maintenance and proliferation of ENStem-ATM Human Neural Progenitor Cells.

Application

Cell culture protocols for use with ENStem-A Human Neural Progenitor Cells are available in the datasheet that accompanies the ENStem-A Human Neural Progenitor Expansion Kit (Cat. No. SCR055).

Components

ENStem-A Neural Expansion Medium, 500 mL (Part No. SCM004a)

FGF-2, 50ug, lyophilized (Part No. GF003)

Quality

Each lot of medium is tested for the optimal cell growth and proliferation of ENStem-ATM Human Neural Progenitor Cells.
Sterility Testing: Negative

Physical form

ENStem-ATM Neural Expansion Medium contains neurobasal medium, B27 supplement, and LIF.

Storage and Stability

ENStem-ATM Neural Expansion Medium should be stored at -20°C until ready to use. Upon thawing, fresh L-Glutamine (not provided) should be added for a final concentration of 2 mM to the expansion medium. Thawed should be stored at 2-8°C and given a 1-month expiration dating. Dispense into aliquots to avoid repeated heating prior to each use.

FGF-2 (10 μg, lyophilized) should be reconstituted with 100 μL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 μg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

wgk_germany

WGK 1


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 1

1 of 1

Da-Jeong Chang et al.
Experimental & molecular medicine, 45, e53-e53 (2013-11-16)
The transplantation of neural precursor cells (NPCs) is known to be a promising approach to ameliorating behavioral deficits after stroke in a rodent model of middle cerebral artery occlusion (MCAo). Previous studies have shown that transplanted NPCs migrate toward the

Articles

Fibroblast growth factors (FGFs) regulate developmental pathways and mesoderm/ectoderm patterning in early embryonic development.

Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service