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SCM005

Sigma-Aldrich

ReNcell NSC Maintenance Media

ReNcell NSC Maintenance Medium is a defined serum-free, growth factor-free neural stem cell medium that has been optimized for the growth & in vitro differentiation of ReNcell immortalized human neural progenitor cells.

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Synonym(s):
ReNcell Neural Stem Cell Media
eCl@ss:
32160801
NACRES:
NA.75

Quality Level

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type neural stem cell(s)

shipped in

dry ice

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This Item
SCM08314372CSCM007
ES2N Basal Medium The ES2N Basal Medium is a defined serum-free neural basal medium.

Sigma-Aldrich

SCM083

ES2N Basal Medium

Stemline® XF MSC Supplement Serum-free, liquid, sterile-filtered, suitable for cell culture

SAFC

14372C

Stemline® XF MSC Supplement

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | stem cell: suitable

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

Quality Level

100

Quality Level

300

Quality Level

500

Quality Level

100

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

input

sample type neural stem cell(s)

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type neural stem cell(s)

input

-

input

sample type neural stem cell(s)

General description

ReNcell NSC Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell immortalized human neural progenitor cells. When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell immortalized VM and CX neural stem cells. Withdrawal of the growth factors from ReNcell NSC Maintenance Medium will result in the spontaneous differentiation of ReNcell immortalized neural progenitor cells

Physical form

ReNcell NSC Maintenance Medium contains DMEM/F12, HEPES, L-glutamine, human serum albumin, human transferrin, putrescine dihydrochloride, human recombinant insulin, L-thyroxine, Tri-iodo-thyronine, progesterone, sodium selenite, heparin, corticosterone. The media does not contain antibiotics.

Storage and Stability

SCM005, ReNcell Neural Stem Cell Maintenance Medium should be stored at -20°C until ready to use. Upon thawing, the maintenance medium should be stored at 2-8°C and given a 1-month expiration dating.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


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Yen-Ju Chen et al.
Genome research, 30(3), 375-391 (2020-03-05)
Circular RNAs (circRNAs), a class of long noncoding RNAs, are known to be enriched in mammalian neural tissues. Although a wide range of dysregulation of gene expression in autism spectrum disorder (ASD) have been reported, the role of circRNAs in
Transcriptional signature and memory retention of human-induced pluripotent stem cells.
Marchetto, Maria C N, et al.
Testing, 4, e7076-e7076 (2009)
Yuyu Song et al.
Scientific data, 6, 190016-190016 (2019-02-20)
The immortalized human ReNcell VM cell line represents a reproducible and easy-to-propagate cell culture system for studying the differentiation of neural progenitors. To better characterize the starting line and its subsequent differentiation, we assessed protein and phospho-protein levels and cell
Se Hoon Choi et al.
Nature, 515(7526), 274-278 (2014-10-14)
Alzheimer's disease is the most common form of dementia, characterized by two pathological hallmarks: amyloid-β plaques and neurofibrillary tangles. The amyloid hypothesis of Alzheimer's disease posits that the excessive accumulation of amyloid-β peptide leads to neurofibrillary tangles composed of aggregated
B J Carroll
Advances in biochemical psychopharmacology, 39, 179-188 (1984-01-01)
The DST is a practical laboratory test that can be used in outpatient or inpatient settings. The required laboratory technology is available at any hospital. No subjective, behavioral, or metabolic side-effects occur with the single-dose administration of dexamethasone, and the

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Protocols

Protocol Guide: Differentiation of Human Neural Stem Cells using TrueGel3D® HTS Hydrogel Plates

Learn to culture ReNcell® human neural stem cells (NSCs) in 3D hydrogels like the TrueGel3D® HTS Hydrogel Plate using this step by step protocol for high-throughput screening cell culture applications.

Alzheimer’s In A Dish™: 3D Neural Stem Cell Models of Alzheimer's Disease

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

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