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GF003AF

Sigma-Aldrich

Fibroblast Growth Factor basic, human recombinant, animal-free

The Fibroblast Growth Factor-basic (or bFGF protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells.

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Synonym(s):
FGF-2, bFGF, basic-FGF, Heparin-Binding Growth Factor 2, Prostatropin
eCl@ss:
32160405
NACRES:
NA.75

biological source

human

Quality Level

Assay

>95% (SDS-PAGE)

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

impurities

<0.1 EU/μg Endotoxin (of bFGF)

input

sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)

NCBI accession no.

UniProt accession no.

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This Item
GF321H6666F5392
assay

>95% (SDS-PAGE)

assay

-

assay

>95% (SDS-PAGE)

assay

~90% (SDS-PAGE and N-terminal analysis)

technique(s)

cell culture | stem cell: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg Endotoxin (of bFGF)

impurities

<0.1 μg/mg protein endotoxin (<1 EU/μg)

impurities

<1 EU/μg

impurities

<0.5 EU/vial

UniProt accession no.

P09038

UniProt accession no.

P05230

UniProt accession no.

P21781

UniProt accession no.

P03969

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

200

General description

Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 155 amino acid residues. Xu, et al. demonstrated that bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions. GF003AF was developed without animal-based ingredients and can be used for the culture of hES cells in a feeder-free, animal-free culture system.
Product Source: expressed in E. coli

Application

For most in vitro applications, bFGF exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL. Responding cells are (partial list): Endothelial, mesenchymal cells. Human ES cells require concentrations in the range of 4 to 100 ng/ml, depending on the method of culture

Physical form

Lyophilized from a solution of 5mM Tris, pH 7.6 with 150 mM NaCl.

Storage and Stability

Maintain the lyophilized material at -20°C until expiration date as stated on the label.

General applications:
After a quick spin, reconstitute in 0.1M phosphate buffer, pH 6.8, to a concentration of 0.1-1.0 mg/mL. Reconstituted bFGF should be stored in working aliquots at -20°C for up to six months. Multiple freeze/thaw cycles will result in significant loss of activity.

For Human ES cell culture:
After a quick spin, reconstitute to 10 μg/mL in a filtered solution of 0.5% BSA, 1 mM DTT, and 10% glycerol in Dulbecco′s PBS. Aliquot and store at -20°C for up to six months. This solution can then thawed and diluted to 4 ng/mL for the culture of human ES cells with a feeder layer, or to 8 ng/mL to supplement mouse embryonic fibroblast-conditioned medium (for feeder-free human ES cell culture).

Analysis Note

Specific Activity: Human FGF-b is fully biologically active when compared to standards. The ED50 was determined by the dose-dependent stimulation of thymidine uptake by NIH3T3 cells expressing FGF receptors.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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Protocols

Learn to culture ReNcell® human neural stem cells (NSCs) in 3D hydrogels like the TrueGel3D® HTS Hydrogel Plate using this step by step protocol for high-throughput screening cell culture applications.

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