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SRP3027

Sigma-Aldrich

EGF human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

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Synonym(s):
Epidermal Growth Factor, URG, Urogastrone
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.2 ng/mL

mol wt

6.2 kDa

packaging

pkg of 500 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... EGF(1950)

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Sigma-Aldrich

Sigma-Aldrich

SRP3027

EGF human

hEGF EGF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sigma-Aldrich

E9644

hEGF

VEGF human Animal-component free, recombinant, expressed in E. coli, &#8805;98% (SDS-PAGE), &#8805;98% (HPLC), suitable for cell culture

Sigma-Aldrich

SRP3182

VEGF human

Sigma-Aldrich

Sigma-Aldrich

SRP6014

HGF human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in CHO cells

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥97% (HPLC), ≥97% (SDS-PAGE)

form

lyophilized

form

lyophilized powder

form

lyophilized

form

lyophilized

mol wt

6.2 kDa

mol wt

~6 kDa

mol wt

38.2 kDa

mol wt

80.017 kDa

packaging

pkg of 500 μg

packaging

pkg of 5X0.2 mg, pkg of 0.2 mg, pkg of 0.5 mg

packaging

pkg of 10 μg

packaging

pkg of 1 mg, pkg of 10 μg

General description

The gene EGF (epidermal growth factor) is mapped to human chromosome 4q25-27. The EGF gene encodes for a large type I membrane–anchored precursor protein which is cleaved into pro-EGF for generation of peptide hormone EGF. The mRNA is expressed in kidney, salivary gland, cerebrum and prostate. Recombinant human EGF is a 6.2kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds.

Application

EGF (epidermal growth factor) human has been used in the culture medium for the maintenance of MCF10A cells (human mammary epithelial cells).

Biochem/physiol Actions

EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). Mutation in the EGF gene might be associated with risk of non- small cell lung adenocarcinoma. It is also involved in anaplastic thyroid cancer and renal hypomagnesemia. It controls cell differentiation and neurotrophic processes.

Sequence

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Clinical Implication of EGF A61G Polymorphism in the Risk of Non Small Cell Lung Adenocarcinoma Patients: A Case Control Study.
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Basal p21 controls population heterogeneity in cycling and quiescent cell cycle states.
Overton KW
Proceedings of the National Academy of Sciences of the USA, 111, E4386-E4393 (2014)
Epidermal growth factor therapy and wound healing--past, present and future perspectives.
Hardwicke J
The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland, 6, 172-177 (2008)
Reactive oxygen species (ROS) are essential mediators in epidermal growth factor (EGF)-stimulated corneal epithelial cell proliferation, adhesion, migration, and wound healing.
Huo Y
Experimental Eye Research, 89, 876-886 (2009)
Neurotrophic actions of a novel molluscan epidermal growth factor.
Hermann PM
The Journal of Neuroscience, 20, 6355-6364 (2000)

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