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LMO001

Sigma-Aldrich

MULTI-seq Lipid-Modified Oligos

for Single Cell and Single Nucleus Multiplexing

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Synonym(s):
LMO, Lipid modified oligos

Quality Level

form

liquid

usage

sufficient for 100 sample(s) (100RXN kit is adequate for labelling 100 samples when using 1 μL of each LMO per sample)
sufficient for 30 sample(s) (30RXN kit is adequate for labelling 30 samples when using 1 μL of each LMO per sample)

concentration

50 μM (each)

technique(s)

multiplexing: suitable (Single Cell and Single Nucleus)

shipped in

dry ice

storage temp.

−20°C

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This Item
F4928F5178SAB4503413
concentration

50 μM (each)

concentration

-

concentration

-

concentration

~1 mg/mL

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

usage

sufficient for 100 sample(s) (100RXN kit is adequate for labelling 100 samples when using 1 μL of each LMO per sample)

usage

-

usage

-

usage

-

General description

MULTI-seq, multiplexing using lipid-tagged indices (lipid-modified oligonucleotides), is a novel tool that facilitates multiplex single-cell and single-nucleus RNA sequencing. This technology provides sample barcoding that is compatible with any cell type or nucleus containing an accessible plasma membrane and allows for streamlined, pooled single-cell sample processing. Learn more about the experiments and data by reviewing our technical article – (MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing)

Features and Benefits

  • Efficient. MULTI-seq increases the throughput and decreases the reagent cost of droplet-based single-cell RNA sequencing.
  • Flexible. Compatible with any droplet generation platform, the indexing system enables users to run and analyze up to 96 barcoded samples simultaneously in an 8-lane droplet device.
  • Clearer Results. The indices provide data quality advantages over non-indexed analysis methods in the form of doublet identification and retention of data from cells with low RNA content.

Components

The MULTI-seq Lipid Modified Oligos kit is comprised of a lignoceric amide-modified anchor DNA oligo solution and a palmitic amide-modified co-anchor DNA oligo solution. Together, these lipid-modified oligos embed into cell or nuclei membranes and provide a landing pad for DNA barcodes with a complementary 5’ sequence. The MULTI-seq Lipid Modified Oligos kit is intended for upstream sample preparation only, before pooling and single cell analysis.

Reagents Provided


  • LMO001A Lignoceric Anchor with DNA Oligo
  • LMO001B Palmitic Co-anchor with DNA Oligo
Each lipid-modified oligo is supplied at a concentration of 50 μM in water.

Other Notes

  • This product is for R&D use only. Not for drug, household, or other uses.
  • Unique barcode oligos are not included and must be purchased separately.
  • Barcode 3’ oligo design with poly-A tail for mRNA enrichment: 5′-CCTTGGCACCCGAGAATTCCA-8-base index-A30-3′
  • Barcode oligo design for 5’ cDNA library synthesis: 5’-CCTTGGCACCCGAGAATTCCA-8-base indexCCCATATAAGAAA-3’
  • In addition, 96 x 5′-end and 96 x 3′-end unique barcode oligos are available to purchase separately. Details on our custom barcode product, Next-Gen Sequencing Oligos (NGSO), can be found at SigmaAldrich.com/nextgenoligos. For minimal cross-contamination of barcode primers, order NGSO-Silver or preferably, NGSO-Gold quality. Please review the specifications available and then submit a quote request for the MULTI-seq barcodes to dnaoligos@milliporesigma.com. Sequences are not provided prior to purchase but will be included on product documentation at the time of delivery.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


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Christopher S McGinnis et al.
Nature methods, 16(7), 619-626 (2019-06-19)
Sample multiplexing facilitates scRNA-seq by reducing costs and identifying artifacts such as cell doublets. However, universal and scalable sample barcoding strategies have not been described. We therefore developed MULTI-seq: multiplexing using lipid-tagged indices for single-cell and single-nucleus RNA sequencing. MULTI-seq

Articles

Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.

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